Thunder TR-FRET signaling assays: now you have an alternative
Why choose TR-FRET assays from BioAuxilium?
Better components
- Carefully valdiated antibodies
- Optimal donor/acceptor fluorophores
- Optimized assay components (e.g. buffers)
Rigorous validation
- All kits are validated on lysates from activated cells
- Technical datasheets contain validation data
Lot-to-lot consistency
- Kits are developed and validated in-house
- New lots are measured against existing lots to ensure lot-to-lot consistency
Affordability
- A superior, cost-effective alternative to ELISA kits
- 30% cheaper than TR-FRET kits from big suppliers
The Thunder TR-FRET assay platform
The ThunderTM TR-FRET assay platform is based on the use of donor-acceptor fluorophores showing exceptional spectral compatibility and TR-FRET signal:
- A long-lifetime and highly stable Europium chelate (Eu) as the donor fluorophore
- A small, unique far-red fluorophore (FR) as the acceptor
In a sandwich immunoassay application, one antibody is labeled with the Eu chelate and the second antibody is labeled with the FR fluorophore. The binding of the two labeled antibodies to distinct epitopes on the target protein takes place in solution and brings the two fluorophores into close proximity. Excitation of the donor Eu triggers a FRET to the acceptor FR, which in turn emits a specific TR-FRET signal at 665 nm. Residual (non-transferred) energy from the Eu generates light at 615 nm. These long-lived fluorescent signals are read in a time-resolved manner to minimize assay interference, which increases assay performance and specificity.
Validation data
Comparison of BioAuxilium TR-FRET ERK1/2 assay kit against Competitor C. Stimulation of phospho-ERK1/2 (T202/Y204) in HEK293 cells.
Kit options
Phospho-protein
Measure the relative amounts of a specific phosphorylated target protein.
Total protein
Measure the relative amounts of a target protein regardless of its phosphorylation status. Useful for monitoring protein expression levels and for normalization purposes.
Phospho-protein and total protein
Provides a novel opportunity to measure matched phosphorylated and total proteins from separate wells in the same plate.
Kit sizes
Enjoy total flexibility: most kits are available as 100, 500, 2500 and 5000 assay point sizes.
Assay workflow
All it takes is three simple steps to complete the workflow of all TR-FRET assays kits from BioAuxilium. Assays can be run using a 2-plate (transfer) or 1-plate (all in one well) protocol.
