Analyze gene expression with PrimeTime® qPCR Assays
PrimeTime qPCR Probe Assays
PrimeTime qPCR Probe Assays are 5′ nuclease assays that include a predesigned assay catalog for human, mouse, and rat sequences. Custom assays may be created for all species using the PrimerQuest Tool. Predesigned and custom assays can be provided in tubes or plates.
Features:
- Predesigned sequences will achieve 90% efficiency or better, or we will replace with an alternative design free of charge
- Select from a wide range of license-free dyes, including FAM, TET, SUN™, HEX and Cy® 5 (Cytiva) dyes
- Multiplex with confidence using double-quenched ZEN™ or TAO™ probe assays
- Select a primer: probe ratio from 2:1 to 4:1 with no additional charge
- Size and scale options available for digital PCR (dPCR) platforms
- Receive primers and probe sequences with all orders
An unbeatable offer: twice the amount of reactions at half the price of a TaqMan assay!
| Supplier | Size (reactions) | List price (CHF) |
|---|---|---|
| Integrated DNA Technologies (PrimeTime probe assay) | 500 | 200.00 |
| ThermoFisher (TaqMan probe assay) | 250 | 386.00 |
More about PrimeTime qPCR Probe Assays
PrimeTime qPCR Primer Assays
PrimeTime qPCR Primer Assays for use with intercalating dyes, such as SYBR® Green dyes, include predesigned assays for human, mouse, or rat sequences. Custom assays may be created for all other species using the PrimerQuest Tool. Predesigned and custom assays can be provided in tubes or plates.
More about PrimeTime qPCR Primer Assays
Custom qPCR probes
Use custom probes to increase signal, decrease background, improve accuracy, or otherwise leverage the broad range of sequence design, dye, and purification options available.
Custom qPCR probes are made to your specifications and include several probe types, including PrimeTime double-quenched probes, Affinity Plus LNA Probes, and molecular beacon probes. Additionally, MGB Eclipse® Probes and other probe types are available as GMP-compliant reagents.
How can I order PrimeTime qPCR assays?
Where can I learn more about PrimeTime® qPCR assays and probes?
Genotyping with rhAmp®
The rhAmp® SNP genotyping system: accurate, affordable genotyping with the next evolution of PCR
- Generate the highest level of performance with greater than 99.5% call accuracy for over 90% of assays tested
- Interrogate SNPs in difficult sequence regions with amplicon lengths as short as 40 bp
- Validate markers affordably using the smallest pack size commercially available
- Ensure confidence in your data with gBlocks® Gene Fragments as control templates
The rhAmp SNP portfolio includes all components needed to successfully generate high quality genotyping data on any commonly available real-time PCR instrument.
Superior chemistry for SNP detection
Superior discrimination versus traditional methods. Blocked primers minimize non-specific amplification. The 3' end of rhAmp primers incorporate a blocking group that prevents extension unless cleavage and de-blocking occur by RNase H2 enzyme. RNase H2 enzyme recognizes this RNA base only if it is hybridized to its perfect complement, initiating primer cleavage and activation.
Ordering information and resources
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.