Chemistries and instrumentation for customers to use in their own laboratories. Allowing highly flexible, and individual solutions for each of our customers. To provide the most efficient and effective extraction or purification method, we offer not only a range of different technologies, but also the capability to develop tailor-made kit systems for all applications and downstream requirements. Our range of technologies allows delivery of optimised nucleic acid extractions and purifications (e.g. genomic DNA, plasmids, RNA) from an unrivalled array of matrices.
Selected top products:
| QuickExtract DNA Extraction Solution | MasterPure™ Complete DNA and RNA Purification Kit | Other Solutions |
| Read more below | Read more below | Contact us |
QuickExtract™ DNA Extraction Solution
Simple, rapid extraction of PCR-ready DNA for screening and genotyping type applications from hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells and other sample types.
Key features
- Simple, rapid extraction of PCR-ready DNA for screening and genotyping type applications
- Fast: 8 minute extraction protocol for most sample types
- Simple: No centrifugation steps of spin columns used to help increase yields
- Automation-friendly: Simple protocol integrated easily into automated workflows
- Safe: Uses only non-toxic reagents
- Recommended for rapid, easy sample prep for T7E1 CRISPR mutation detection assays
For DNA screening and genotyping applications, PCR-grade genomic DNA is sufficient, and can be rapidly and efficiently extracted using QuickExtract DNA Extraction Solution. In 3-8 minutes, and using only a single-tube protocol, PCR-grade genomic DNA or RNA from almost any sample type can be extracted, without the use of centrifugation, spin columns or any toxic organic solvent.
The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes (Fig. 1), depending on the sample. QuickExtract Solution has been used to extract DNA from samples such as hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells, zebrafish organs and scales, and mouse tail snips. The extracted DNA is suitable for PCR analyses (Figure. 2), such as genomic, transgenic, or viral DNA screening in animals, or for genetic or environmental research and screening in humans and other organisms.
Applications
- Produce PCR-ready DNA for transgenic mouse genotyping, genetic studies, human identity testing, or viral/microbial screening.
The QuickExtract method allows for the inexpensive processing of one to hundreds of samples simultaneously, without centrifugation, spin columns or the use of any toxic organic solvent. The method is also compatible with robotic automation.
using QuickExtract DNA Extraction Solution.
FailSafe™ PCR amplification of genomic DNA extracted using QuickExtract
Buccal cells were extracted using the BuccalAmp™ DNA Extraction Kit, and all other samples with QuickExtract™ DNA Extraction Solution. PCR was performed using primers to amplify the regions indicated: Lanes 1-3, human β-globin; lane 4, transgenic mouse GAPDH; lane 5, E. coli 16S ribosomal RNA gene; lane 6, transgenic SV40 T antigen.
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| QE09050 | QuickExtract DNA Extraction Solution 1.0 | 50 ml | 512.00 |
Documentation
| Filename | Download |
|---|---|
| E103-US-QuickExtract_lub.pdf |  |
| MA150E-QuickExtract-DNA-Solution.pdf | |
MasterPure™ Complete DNA and RNA Purification Kit
Quickly purify high yields of high-molecular-weight genomic DNA, total cellular RNA or Total Nucleic Acid (TNA) with one kit.
Key features
- Purify Total Nucleic Acid (TNA), DNA or RNA in 30-60 minutes
- Safe: Does not use hazardous phenol, chloroform or guanidine
- High Purity: A260/A280 ratios consistently between 1.8 and 2.0
- High Yields: Improves yields by avoiding the use of columns which often reduce nucleic acid yields
- Versatile: Purify TNA, genomic DNA, total RNA, FFPE RNA, or both genomic DNA and total RNA from a sample
- Total RNA Recovery: Purify both large and small (e.g., miRNA) RNA for RNA-Seq or qRT-PCR
- Proven: Hundreds of citations for purification of DNA and RNA from dozens of sample types for use in many applications
Some applications require flexibility of isolating high-molecular weight genomic DNA, total cellular RNA or total nucleic acid from the same sample. The MasterPure Complete DNA and RNA Purification Kit allows for isolation of NGS-grade DNA, RNA or total nucleic acid in 30-60 minutes using a single kit, and is suitable for many different sample types.
The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
The kit procedure uses a safe, gentle, and scalable salt-precipitation protocol (Figure. 1) that eliminates the need for hazardous chemicals and yield-reducing columns. Purification reactions can be scaled up or scaled down depending on the amount of nucleic acid needed and the size of the sample. MasterPure Kits for nucleic acid purification from Gram-positive bacteria, yeast (DNA or RNA), and blood samples are also available.
Applications
Purification of genomic DNA or total RNA for many applications, including:
- Library preparation for next generation sequencing (NGS) of genomic DNA and RNA
- DNA methylation studies using Illumina® Infinium® HumanMethylation BeadChips
- Genomic DNA and cDNA cloning
- qPCR and qRT-PCR
- Micrarray analyses (CGH, gene expression profiling, etc)
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| MC89010 | MasterPure Complete DNA & RNA Purification Kit | 10 purifications | 141.00 |