Blocking reagents for western blotting

SureBlock - the high capacity blocking agent for Western blotting

SureBlock™ is a bovine gelatine-based blocking agent for various applications, including Western blotting. Compared to BSA, it is more economical, has a higher blocking capacity and less background.

SureBlock - applications, advantages and info

Applications

Western blot / Dot blot
ELISA
Immunohistochemistry (IHC)
Surface coating
Gene-chip Technology (replacement of acetylated BSA)

Advantages

Better blocking capacity than BSA
Virtually no background in Western blotting
Increased signal-to-noise ratio in ELISA
Solubility guaranteed up to 50% [w/v] at 4°C
More economical than BSA

SureBlock™ is a replacement for our previous TopBlock products (TB232010) and is identical in chemical composition and formulation.

Citations

Publication year	Title	Journal
2021	The role of macrophage scavenger receptor 1 (Msr1) in prion pathogenesis.	Journal of molecular medicine
2020	Ribosomal profiling during prion disease uncovers progressive translational derangement in glia but not in neurons.	eLife
2020	Prion infection, transmission, and cytopathology modeled in a low-biohazard human cell line.	eLife
2020	Soluble dimeric prion protein ligand activates Adgrg6 receptor but does not rescue early signs of demyelination in PrP-deficient mice.	PloS One
2017	Prion pathogenesis is unaltered in the absence of SIRPα-mediated "don't-eat-me" signaling.	PloS One
2015	Prion infections and anti-PrP antibodies trigger converging neurotoxic pathways.	PloS Pathogens
2012	Prion pathogenesis is faithfully reproduced in cerebellar organotypic slice cultures.	PloS Pathogens

Download manual Download MSDS

Cat-No.	Item	Size	Price (CHF)
SB232010-250G	SureBlock - blocking agent for Western Blotting	250 g	199.00
SB232010-500G	SureBlock - blocking agent for Western Blotting	500 g	299.00