Explore FuGENE market-leading DNA transfection reagents for delivery of DNA into eukaryotic and insect cells. FuGENE® 4K, FuGENE® 6 and FuGENE® HD combine high-efficiency transfection with extremely low toxicity to your cells. Whether it’s for life science research, protein/antibody production, virus production, or for groundbreaking therapeutic needs, FuGENE® has you covered.
Why FuGENE?
- The world leader in mammalian transfection - over 25 years of experience
- Wide range of transfection reagents for primary cells & cell lines
- Thousands of publications and reference protocols for virtually any cell type
Which FuGENE reagent is the right one for me?
- FuGENE 4K - best-in-class reagent for highest transfection efficiencies and difficult to transfect cells
- FuGENE HD - for protein and virus production, delivery into primary and stem cells
- FuGENE 6 - affordable, reliable transfection of most common cell lines
- FuGENE SI - superior delivery of siRNAs, miRNAs, short RNA molecules
Discover the gentle magic of FuGENE
FuGENE 4K
- Outperforms Lipofectamine® 3000 in most cell types
- Most effective broad spectrum DNA transfection reagent
- Maximum transfection efficiency in adherent cells
- Robust performance in suspension adapted 293 & CHO cells
- Improved viral titers and protein production yields
Key benefits
- Transfect in serum-free or serum-containing media
- Improved performance in the most relevant cancer cell lines
- Improved viral titer and capsid fullness in AAV/LV systems
- Maximizes protein production in 293 & CHO cells
FuGENE® 4K reagent outperforms Lipofectamine® 3000. A549, C2C12, HCT116, CHO-K1, HeLa, NIH3T3, LNCaP, PANC1 & SKOV3 cell lines were seeded in 96-well plates according to user guides and subsequently transfected with FuGENE® 4K or Lipofectamine® 3000 (registered trademark of Thermo Fisher Scientific) and a GFP expressing plasmid at the following transfection reagent to DNA ratios (3:1, 4:1 & 5:1). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % GFP expressing cells and % total cell viability (via propidium iodide dye). The graphs above show the superior performance of FuGENE® 4K vs. Lipofectamine® 3000.
Product literature
| Filename | Download |
|---|---|
| FuGENE_4K-Users-Guide_2022_REV1.3.pdf |  |
| FuGENE_4K-Optimization-Guide_2022.pdf | |
FuGENE HD
- Transfection of difficult-to-transfect cell lines
- Superior efficiency with minimal-to-no toxicity
- Maximizes protein, antibody, and virus production
- Your choice for primary and stem cells
Key benefits
- High transfection efficiency proven in more than 1000 cell types, including many “hard-to-transfect” cells
- Minimal to no toxicity
- Transfect in serum-free or serum-containing media
- Easy to use - simply dilute FuGENE® HD, incubate with plasmid DNA, and add directly to your cells
Product literature
| Filename | Download |
|---|---|
| FuGENE_HD-User-Guide.pdf | |
| FuGENE_HD-Optimization-Guide.pdf | |
FuGENE 6
- The standard transfection reagent for every lab
- Cost-effective option for most cell lines
- Over 1000 cell types successfully transfected
- High efficiency transfection with minimal toxicity
Key benefits
- High transfection efficiency proven in more than 800 cell types, including many “hard-to-transfect” cells
- Generate physiologically relevant results with the low-cytotoxicity of FuGENE 6®
- Use less reagent per transfection than with competiror's transfection mixes
Product literature
| Filename | Download |
|---|---|
| FuGENE_6-User-Guide.pdf | |
| FuGENE_6-Optimization-Guide.pdf | |
FuGENE SI
- Maximum efficiency & gentle siRNA transfection reagent
- Utilize lower amounts of transfection reagent and siRNA
- Superior gene silencing with minimal-to-no toxicity
- Less toxic and more efficient than RNAiMax®
Key benefits
- High-efficiency transfection of siRNAs into eukaryotic cells
- No cell toxicity or slowing of growth rate
- Superior knockdown efficiencies with lower amounts of siRNAs
- Transfect in serum-free or serum-containing media
HEK293-GFP & NIH3T3-GFP Cell lines (Cell Biolabs®) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0, or 5 pmols of GFP targeting siRNA or negative control (Horizon Discovery/Dharmacon®), along with 0.3ul of Transfection Reagent FuGENE® SI or 0.3ul of Lipofectamine® RNAiMax (Life Technologies Corporation). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % knockdown of GFP & total cell viability. Graphs above show the superior knockdown performance of FuGENE® SI vs. Lipofectamine® RNAiMax in HEK293 and NIH3T3 cells.
Product literature
| Filename | Download |
|---|---|
| FuGENE_SI-User-Guide.pdf | |
| FuGENE_SI-Optimization-Guide.pdf | |
Explore our FuGENE Reference Database
The FuGENE reference database includes thousands of published papers citing usage of FuGENE across 1000’s of different cell lines. Search by key attributes such cell type, application, product line, or even year of publication. Have confidence knowing FuGENE is one of the most widely published transfection reagents in the world!