Next Generation Sequencing (NGS)

Get started by requesting your free consultation with our NGS service experts. We would love to discuss your research objectives and are happy to provide assistance in defining your project goals. We are here to provide customized recommendations to suit your project needs.

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Shipping your samples to our partner's facility is easy and painless! We’ll provide you with detailed instructions on how to handle, process and ship your samples to us. We accept both purified RNA and specimens for RNA isolation services.

If you are shipping specimens to us, a complete guide on sample collection and preservation will be provided to you.

Our partners have tremendous experience and expertise in isolating RNA from ultra-low concentration samples such as liquid biopsies (plasma, serum, urine, CSF), exosomes and FFPE. Utilizing our proprietary silicon carbide resin for all isolations allows them to capture miRNA with greater diversity and without the use of phenol.

You can be confident that the sequencing portion of your project will be successful as our partners perform quality control on the isolated RNA to assess quantity, quality and amplifiability prior to library preparation.

Our partners have expertise in RNA library preparation from all types of specimens, successfully generating libraries from ultra-low RNA inputs. They will generate the small RNA libraries using Norgen Biotek’s Small RNA Library Preparation Kit (for Illumina). The libraries will then undergo a quality check prior to sequencing.

Our partners will sequence the sample libraries on the Illumina® NextSeq platform to achieve your required coverage and read depth.

Once the sequencing is complete, the bioinformaticians conduct a comprehensive analysis to help make sense of your data. Customized advanced analyses, such as Differential Expression, KEGG/GO and Novel miRNA Discovery can be added to the service to answer a specific biological question. You will receive a final report, which will include a summary of your project as well as your data illustrated in ready-to-publish figures.