xGen™ Broad-Range RNA Library Preparation Kit
Comprehensive data from a wide range of RNA inputs
The xGen™ Broad-Range RNA Library Preparation Kit offers a fast, robust NGS transcriptomics workflow with optimal transcript coverage and NGS data quality from a broad range of input quantities for Illumina® sequencing platforms. Leveraging patented Adaptase® technology, this kit enables stranded RNA library construction directly from 1st strand cDNA without the requirement for 2nd strand cDNA synthesis and degradation, or template-switching methods. The kit is compatible with manual and automated workflows as well as upstream and downstream enrichment and depletion methods and supports a variety of indexing options.
- Superior Data: High mapping rates, genes detected, and transcript coverage
- Accommodates your Samples with a Single Kit: Consistent performance from 10 ng – 1 μg total RNA or 100 pg – 100 ng mRNA input
- Robust Performance: Consistent libraries with minimal adapter dimers and no adapter titration required for all supported inputs
- Save Time, Reduce Costs, Automate: Go from RNA to library in 4.5 hours; variety of index options including 96 UDIs and up to 768 combinatorial dual indexing; automation-friendly
xGen™ RNA Library Preparation Kit
Accelerate your RNA-Seq transcriptomics research
The xGen™ RNA Library Preparation Kit offers the fastest, lowest cost NGS transcriptomics workflow for Illumina® sequencing platforms. Leveraging patented Adaptase® technology, this kit enables stranded RNA library construction directly from 1st strand cDNA without the requirement for 2nd strand cDNA synthesis and degradation, or template-switching methods. The kit is compatible with upstream and downstream enrichment and depletion methods, supports a variety of indexing options, and is easily automatable.
- Save time and cost: At 3.5 hours, this is the fastest RNA library kit on the market. Combined with the lower cost, you can process more samples in less time to increase your data output.
- Supports many workflows with indexing options, automation, and Normalase: single, combinatorial dual indexing up to 768, unique dual indexing up to 96, and enzymatic library normalization available; automation-friendly.
- Robust Performance: Maintains ligation efficiency at all supported inputs and no adapter titration required.
Read more about single-cell RNA sequencing on our Blog
Technical note
| Filename | Download |
|---|---|
| IDT_TechNote-tail-trimming-for-better-data.pdf |  |
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.