Don't trash your research – Trash your bad oligos instead
Are you using oligonucleotides in your research? Have you encountered problems such as failed clonings, inefficient ligations, or unspecific PCRs? Watch our 100-second tutorial to understand how oligo quality affects your experiments.
Go for quality - Use oligos from the market leader IDT
Integrated DNA Technologies (IDT) synthesizes its oligos on proprietary synthesis platforms and automation, using optimized phosphoramidite chemistry and high-quality chemicals for sustainable manufacturing. Therefore, IDT oligos are of top quality and contain fewer incomplete or truncated oligos. IDT's offerings encompass primers for PCR, dual-labeled probes for qPCR, indexed adapters and fusion primers for sequencing, and a variety of custom products. They also offer a vast range of oligo modification options, including fluorophores, dark quenchers, modified bases, linkers, spacers, click chemistry, and more.
All Benefits of IDT Oligos at a Glance:
- Highest coupling efficiency in the industry
- Every oligo is fully QC'd by ESI-MS1
- Full-length oligos - no need for HPLC purification
- More than 500 modifications available
- Free 2-3 day delivery via DHL
1 With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI -MS.
Custom formulations
Available modifications
| Description | Examples | Applications | |
|---|---|---|---|
| Attachment chemistry and linkers | Link an oligo with another molecule or particular surface. | Amino modifiers, Biotin, Thiol, Alkynes | DNA arrays, solid-phase PCR, NGS |
| Fluorophores and dark quenchers | Fluorescent dyes re-emit light upon excitation while dark quenchers absorb the emitted light and release heat. | Fluorescein Dyes, Cy Dyes, Rhodamine Dyes, ATTO dyes, Alexa Dyes, LI-COR Dyes, Iowa Black Quenchers, Black Hole Quenchers | qPCR, dPCR, for gene expression or genotyping |
| Freedom dyes | Have no patent licensing restrictions. | FAM, SUN, Cy 5 | qPCR, dPCR, for gene expression or genotyping |
| Modified bases | A variety of modifications that can serve a range of functions, including cross-linking, duplex stabilization, and nuclease resistance. | Affinity Plus: +A, +G, +C, +T O-Methyl: mA, mG, mC, mU RNA: rA, rG, rC, rU | qPCR, dPCR, antisense Pending the modification can be used to adjust Tm, increase resistance to nuclease degradation, reduce toxicity/limit unwanted immune responses |
| Phosphorylation | Use if your oligo is being used as a substrate for DNA ligase. 3’ phosphorylation will inhibit degradation by some 3’ exonucleases and can be used to block extension by DNA polymerase. | 5’ or 3’ Phos | qPCR, dPCR for genotyping, Synthetic biology applications, NGS |
| Spacers | Create distance between a functional moiety and the hybridizing region of your oligo. | C3 Spacer, Spacer 18, Hexanediol | qPCR, dPCR for genotyping, Synthetic biology applications, molecular applications where bulky mods are attached to oligos (like Cholesterol) |
| Phosphorothioate bonds | Include these bonds in your oligo sequence for increased inter-nucleotide resistance to nuclease degradation. These modified bonds are especially beneficial when incorporated into antisense oligos. | *A, *G, *C, *T | Antisense, FISH probes, and other cellular mapping |
Further options and services
Add purification to increase success in demanding applications such as multiplex PCR, cloning, mutagenesis, or antisense/RNAi methods. Purification services offered include PAGE and various types of HPLC.
IDT offers optional QC services to suit your particular product needs. Purified oligos up to 60 bases that are provided with a purity guarantee receive QC by capillary electrophoresis (CE).
If you are in a hurry, IDT offers various expedited services.
Free Trial for New Customers
Need more data?
Look at our side-by-side comparison of oligo quality
- 32 oligos of different lengths synthesized either by IDT or by a direct competitor
- Oligos were analyzed by capillary electrophoresis to determine the percentage of full-length product in each tube
- IDT: average 81% full-length product, competitor: average 54% full-length product
- More than half of competitor's oligos would have been rejected by IDT's quality control!
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.