FPLC (Fast Protein Liquid Chromatography) is a liquid chromatography that is often used to analyze or separate compounds from a mixture of proteins. In FPLC, the mobile phase is an aqueous solution or buffer. The stationary phase is a resin contained in a column. The sample is introduced into the column and migrates into the resin following the flow of the liquid phase. The different components of the sample will then bind or diffuse into the resin. There are several types of FPLC depending on the resin used. Compounds can be separated by their size (size exclusion chromatography (SEC)), by their charge distribution (ion exchange chromatography) or by their biological or chemical affinity for the resin used (affinity chromatography).
For ease of use, a wide range of pre-filled columns for techniques such as ion exchange, gel filtration (size exclusion), hydrophobic interaction and affinity chromatography are available. It is also possible to opt for empty columns in order to fill them with the resin of one's choice.
Protein Ark’s HiFliQ® 1 ml and 5 ml FPLC columns come pre-packed and ready for use with pre-charged Heparin, Nickel-NTA, Cobalt-NTA and Glutathione Agarose resin for affinity purification of Heparin-binding protein, poly-histidine and GST-tagged recombinant proteins. We also offer Protein A & G HiFliQ® FPLC columns, and Q-type & S-type HiFliQ® FPLC columns pre-packed and ready to use for simple, one-step and rapid antibody purification.
These columns are compatible with all common chromatography instruments (including AKTA™ FPLC’s) and a wide range of reducing agents, detergents and other buffer additives. Our Heparin, Nickel-NTA, Cobalt-NTA and Glutathione Agarose chemistries are coupled to HP resin to ensure high selective binding capacities.
HiFliQ® FPLC Columns
Purification by immobilized metal ion affinity chromatography (IMAC)
Protein Ark HiFliQ columns pre-packed and ready to use with pre-charged Nickel-NTA and Cobalt-NTA Agarose resin for affinity purification of poly-histidine tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). Available in 1 ml and 5 ml HiFliQ column sizes with high binding capacity and minimal ion leakage. Compatible with all common chromatography HPLC and FPLC instruments (including ÄKTA™ FPLC’s), and low pressure pumps and syringes using an appropriate adaptor.
Specifications
- Column volume: 1 or 5 ml resin
- Column construction: Polypropylene
- Resin: Super Ni-NTA Agarose and Super Co-NTA Agarose
- Base Matrix: 7.5% cross-linked Agarose
- Ni-NTA capacity: 50-75 mg (per 1 ml resin) Co-NTA capacity: 40-50 mg (per 1 ml resin)
- Flow rate: 1 ml/min (1 ml), 1-5 ml/min (5 ml)
- Max pressure: 0.5 MPa (72 psi)
- Universal 10.32 (1/16’’) UNF threads: Inlet Female/Outlet Male
- Dimensions: 15 x 80 mm (1 ml), 23 x 80 mm (5 ml)
HiFliQ Co-NTA FPLC Columns
Protein Ark’s HiFliQ Co-NTA FPLC columns designed for rapid one-step purification, and ideal for preparative purification and contaminant removal. HiFliQ Co-NTA FPLC columns are supplied pre-packed and ready to use with pre-charged Cobalt-NTA Agarose resin for affinity purification of poly-histidine tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
The Cobalt-NTA Agarose resin provides high binding capacity with minimal Co2+ ion leakage for high stability, chemical compatibility and reuse. Compatible with all common liquid chromatography instruments (including ÄKTA™ FPLC’s), peristaltic pumps and syringes.
HiFliQ Ni-NTA FPLC Columns
Protein Ark’s HiFliQ Ni-NTA FPLC columns designed for rapid one-step purification, and ideal for preparative purification and contaminant removal. HiFliQ Ni-NTA FPLC columns are supplied pre-packed and ready to use with pre-charged Nickel-NTA Agarose resin for affinity purification of poly-histidine tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
The Nickel-NTA Agarose resin provides high binding capacity with minimal Ni2+ ion leakage for high stability, chemical compatibility and reuse. Compatible with all common liquid chromatography instruments (including ÄKTA™ FPLC’s), peristaltic pumps and syringes.
HiFliQ Ni Advance FPLC Columns
Fastback Ni Advance Resin designed for faster purification of secreted proteins using clarified culture media directly. Available in 1 ml and 5 ml HiFliQ column sizes with high ligand density and high binding capacity. The Fastback Ni Advance resin resistant to EDTA (up to 20 mM) and DTT (up to 20 mM) for IMAC chromatography, simply load your culture media onto the column!
- Ideal for secreted proteins and all intracellular protein expression
- Works in your protocol, regardless of sample origin whether?eukaryotic (insect, yeast, HEK293 or CHO) and bacteria (E.coli)
- Great results faster with fewer steps than conventional workflow – no need to buffer exchange your conditioned media; simply load clarified culture media directly on to the Ni Advance resin column.
- Keep your buffer in its preferred conditions as Fastback Ni Advance resin is resistant to EDTA (up to 20mM) and DTT (up to 20 mM).
Empty FPLC chromatography columns
ÄKTA-compatible columns
Proteus FLiQ are robust empty FPLC chromatography columns that can be filled with any agarose resin (1ml, 5ml, 10ml and 20ml). They are simple to self-pack and compatible with all resin types. Both ends of these columns have 10-32 UNF threads which fit common chromatography instruments such as ÄKTA.
Bed Volumes: FliQ1 FliQ5 FliQ10 FliQ20
- Housing: Polypropylene housing (FliQ1), Acrylic (FliQ5, FliQ10, FliQ20)
- Maximum pressure: 70 psi/5 bar (FliQ1), 42 psi/3 bar (FliQ5, FliQ10, FliQ20)
