For everything from traditional cloning to the assembly of large constructs
gBlocks HiFi Gene Fragments are double-stranded DNA fragments with sizes between 1'000–3'000 bp and verified with a median error rate of less than 1:12'000 via NGS. These high-quality, high-fidelity constructs facilitate the assembly of large and complex sequences, matching both the length and accuracy needed to minimize the introduction of unwanted substitution or deletion errors.
- Clone with confidence: less than 2 colonies need to be analyzed on average to identify an error-free clone
- Flexibility for your projects: HiFi gBlocks are up to 3'000 bp in length
- Ideal for large gene construction, including pathway development and microbe design
With either gBlocks or gBlocks HiFi Gene Fragments, you can easily assemble and clone your DNA fragment into the vector of your choice using a variety of cloning methods, including the Gibson Assembly® method and blunt- or cohesive-end cloning protocols. For added flexibility, you can order gBlocks Gene Fragments with or without a 5′-phosphate group.
High fidelity and purity for gene assembly
IDT gBlocks and gBlocks HiFi Gene Fragments demonstrate high probability of cloning success. With a median error rate of 1:12'000 for gBlocks HiFi Gene Fragments, IDT Gene Fragments demonstrate a high probability of first-time cloning success. Based on data derived from NGS sequencing of gene fragments, gBlocks Gene Fragments and gBlocks HiFi Gene Fragments are up to 45% more likely to give a correct clone the first time when cloning fragments up to 3'000 bps compared to an alternate supplier.
IDT gene fragment data demonstrate improved fidelity versus that from other suppliers
IDT gBlocks and gBlocks HiFi Gene Fragments produce a higher percentage of correct colonies when compared to two other suppliers. Based on screening and sequencing of 24 colonies per sequence, IDT’s fragments were the only fragments to have greater than 75% correct colonies with the desired full-length sequence, and the only gene fragments to achieve greater than 90% correct colonies for fragments that were 1 kb or more in length.
Minimal screening effort needed to find your correct clone
Using IDT gene fragments can reduce the time and expense of screening colonies compared to fragments from other suppliers. Cloning efficiency is affected by many factors, including the cloning method used, the stability of the cell line and plasmid, vector preparation, and toxicity or stress from expression of coding sequences. The values in Table 1 represent typical screening numbers needed when using a seamless cloning method.
Table 1. gBlocks and gBlocks HiFi Gene Fragments reduce the time and expense of screening colonies. The approximate number of colonies to screen for a 90% chance of getting a correct clone.
| Length (bp) | gBlocks Gene Fragments | gBlocks HiFi Gene Fragments |
| ≤ 1'000 | 2 -3 | N/A |
| 1'001 - 2'000 | 3 | 2 |
| 2'001 - 3'000 | 4 | 2 |
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.