Bio X Cell's specialty are high quality monoclonal antibodies for in vivo research. With over 300 monoclonal antibodies covering a diverse range of research areas, Bio X Cell is your source for purifed antibodies. Bio X Cell products are manufactured in an animal-free facility in tissue culture and purified by affinity chromatography. They are ultra-pure with extremely low endotoxin levels and contain no preservatives or stabilizers. Their reagent portfolio is specialized towards antibodies which are widely used for in vivo and in vitro antigen neutralization and pathway blockade as well as cell specific depletion.
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Recombinant in vivo antibodies
Bio X Cell RecombiMAb™ monoclonal antibodies are recombinant antibodies with the exact same antigen-binding variable domains as the traditional antibodies that they are derived from. The epitope to which the RecombiMAb™ antibody binds is identical to that of the matching traditionally produced clone. This is accomplished by sequencing and cloning the antibody-coding genes into high-yield expression vectors which are then expressed in mammalian cells to generate the recombinant antibody.
RecombiMAb™ advantages
- Exceptional Purity Each lot is QC tested for purity using SDS-PAGE and are preservative, stabilizer, and carrier protein-free.
- Binding Validation Each lot of RecombiMAb™ product is validated for antigen binding by immunoblot.
- Ultra-low Endotoxin Levels The level of endotoxin is QC tested for each lot. Our RecombiMAb™ products are ≤ 1EU/mg. If endotoxin levels below 1EU/mg are required, please contact technical support to discuss your needs.
- Pathogen Free Each lot of RecombiMAb™ product is screened for an exhaustive panel of murine pathogens. The results are detailed on product-specific datasheets, to help you adhere to IACUC and Animal Facility requirements.
- Low Protein Aggregation Each lot of RecombiMAb™ is QC tested for aggregate level and guaranteed to be below 5% of the total protein.
Monoclonal in vivo antibodies
Monoclonal antibodies and isotype controls are available in the formats InVivoMab™ and InVivoPlus™. The benefits are
- high purity (> 95%)
- low endotoxin levels < 2 EU/mg (< 1 EU/mg for InVivoPlus™)
- no preservatives or stabilizers added, free of azides
- free of murine pathogens (InVivoPlus™)
- available in bulk sizes
All antibodies are manufactured in-house. Full refund for antibodies that do not work in the stated applications.
InVivoMab™
- Formulated for in vivo application (but can also be used for in vitro)
- > 95% pure, verified via SDS-PAGE
- Low endotoxin levels (< 2 EU/mg)
- Azide and carrier protein free
- If desired, larger quantities possible (mg to g)
- High concentrations (3-10 mg/ml)
If additional QC measures are desired, such as murine pathogen testing and binding validation, see Bio X Cell's InVivoPlus™ antibodies.
InVivoPlus™
InVivoPlus™ antibodies are structurally and functionally identical to the InVivoMab™ versions, with the added benefit of additional QC measures.
- Formulated to exceed the rigorous standards required for in vivo work
- > 95% pure, validated via flow cytometry, ELISA, and/or Western blot
- Ultra-low endotoxin levels (< 1 EU/mg)
- Aggregates validated at ≤ 5% via dynamic light scattering
- Murine pathogen free (screened using ultrasensitive qPCR)
- If desired, larger quantities possible (mg to g)
InVivoSIM antibodies™
Bio X Cell's InVivoSIM™ non-therapeutic antibodies are research grade biosimilar monoclonal antibodies that have the same active biological component as the original therapeutic antibodies. Biosimilars make it possible to study the biological effects of a drug without the need to source an expensive pharmaceutical grade therapeutic. InVivoSIM™ antibodies feature greater than 95% purity and are preservative, stabilizer, and carrier protein-free. InVivoSIM™ antibodies are screened for murine pathogens, screened for protein aggregation, feature binding validation via Immunoblot, and are guaranteed to contain less than 1 endotoxin unit per milligram. InVivoSIM™ antibodies are for Research Use Only and not for therapeutic use.
Isotype controls
Isotype control antibodies serve as negative controls, allowing researchers to differentiate between non-specific background signals and specific effects of the antibody of interest. They must match the species and isotype of the primary antibody of interest but have no specificity for the target species.
- > 95% pure via SDS-PAGE
- Available in InVivoMab™ and InVivoPlus™ formats
- Ready for in vivo administration
- Azide and carrier protein free
- If desired, larger quantities possible (mg to g)
- High concentrations (3-10 mg/ml)
InVivoPure™ dilution buffers
Bio X Cell's InVivoPure™ dilution buffers are specifically formulated to satisfy the stringent requirements of in vivo applications. They identically match the composition and pH of the buffer Bio X Cell's antibodies are formulated in.
- Non-toxic, non-antigenic in animal models
- Ultra-low endotoxin levels (< 0.5 EU/mg)
- Murine pathogen free
ReadyTag™ antibodies and fusion proteins
ReadyTag™ antibodies
Recombinant proteins are often produced in vitro with a small antigenic sequence to which an antibody can bind specifically. The most commonly used of these tag epitopes include polyhistidine tag, HA tag, c-myc tag, as well as proteins like GFP and GST.
- Formulated for in vivo applications
- > 95% purity
- Low endotoxin levels (< 2 EU/mg)
- Preservative, stabilizer, and carrier protein free
Fusion proteins
The fusion proteins are constructed by combining the binding domain of a cell surface receptor with the Fc portion of an antibody. The receptor portion functions as a decoy binding site to bind and capture ligand molecules that would otherwise bind to the endogenous receptor.
- Recombinant antibody (hum/hum)
- Formulated for in vivo applications
- > 95% purity
- Available in InVivoMab™ and InVivoPlus™ formats
- Preservative, stabilizer, and carrier protein free
Documentation
| Filename | Download |
|---|---|
| Bio X Cell - Cancer research antibodies brochure |  |
| Bio X Cell - CD marker antibodies brochure | |
| Bio X Cell - Cell adhesion antibodies brochure | |
| Bio X Cell - Cytokine antibodies brochure | |
| Bio X Cell - Immunology research antibodies brochure | |
| Bio X Cell - Biosimilar antibodies brochure | |
| Bio X Cell - Depletion antibodies brochure | |
