FlexAble is a novel antibody labeling kit that uses an affinity linker to conjugate fluorochromes, enzymes, and molecules in any buffer condition.
- Compatible with primary antibodies from any supplier
- Works with any antibody concentration and formulation
- Fast and easy 2-step protocol – Ready to use in 10 minutes
- Label as little as 0.5ug. No buffer exchange needed
- Label up to 50 different antibodies with 1 kit
- No additional equipment required
How does the FlexAble Antibody labeling kit work?
Antibody labeling with FlexAble is achieved by using a high-affinity, non-covalent FlexLinker. The FlexLinker binds tightly to the antibody within minutes and once bound does not release from the antibody. Unbound FlexLinker is neutralized by adding a FlexQuencher.
The labeling reaction using FlexAble yields a homogenous, well-defined antibody that is fully functional. Neither aggregation nor a loss of antibody is observed during the labeling.
FlexAble is compatible with antibodies from every supplier
FlexAble labeling kits can be used to label:
- Primary antibodies from any supplier
- Antibodies of any concentration
- Antibodies in any buffer formulation
FlexAble Antibody Labeling Kits have been thoroughly validated with primary antibodies from different suppliers. Primary antibodies with low concentration, antibodies stored in 50% glycerol, and antibodies supplemented with carriers like BSA are compatible for use with the FlexAble kits.
See why researchers are choosing FlexAble
Antibody labeling is now faster and easier with our new kits, offering you ultimate flexibility in your workflow. Watch the video to learn more about FlexAble.
FlexAble works in the presence of BSA, glycerol, amine-buffers, and preservatives
The FlexAble Antibody Labeling Kit works with any antibody formulation. Labeling with the high-affinity FlexLinker is not affected by stabilizers like BSA or glycerol, amine-containing buffers, or preservatives.
Antibody buffer formulation
FlexAble Antibody Labeling Kits work with any antibody formulation without the need to purify or buffer-exchange prior to labeling. This saves time and money and also avoids the loss of antibody that can happen during those processes. Since FlexAble utilizes a high-affinity FlexLinker molecule that binds site-specifically to an antibody, the binding reaction is not affected by carriers like BSA or glycerol, amine-containing buffers like Tris, or preservatives like sodium azide.
Simplified muliplexing with FlexAble
CoraLite Plus spectra
CoraLite® and CoraLite Plus dyes are bright fluorescent dyes from Proteintech that deliver high photostability and minimal fluorescent bleed through, making it easy to combine two or more dyes in an experiment. Our FlexAble Kits are conjugated to multiple CoraLite® and CoraLite Plus dyes to offer more flexibility in multiple labeling experiments.
CoraLite and CoraLite Plus dyes have equivalent brightness to the Alexa Fluor® dyes. Each fluorophore can be used simultaneously in co-localization studies due to the minimum overlapping fluorescence spectra. The CoraLite line of FlexAble enables faster and simpler multiplex detection by immunofluorescence, western blotting and flow cytometry.
FlexAble Antibody labeling kits
| Conjugate | Size | Compatible with |
|---|---|---|
| CoraLite® Plus 405 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a NEW |
| FITC Plus | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a NEW |
| CoraLite 488 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a, Mouse IgG2b NEW |
| CoraLite® Plus 555 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a, Mouse IgG2b NEW |
| CoraLite® Plus 647 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a, Mouse IgG2b NEW |
| CoraLite® Plus 750 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a NEW |
Proteintech - The benchmark in antibodies
Primary antibodies, nanobodies, cytokines & growth factors - all made in-house. Strict validation by western blot, ELISA and siRNA testing.