FastGene Western Blot System
Western blot is a molecular biological method for the detection of proteins by transferring them (blotting) onto a carrier membrane. Certain proteins can then be detected through various subsequent reactions.
- Efficient and reproducible protein transfer
- Cooling pack absorbs transfer heat
- Runs two blots simultaneously
Biochemical protein analysis via blotting
Blotting of proteins is a powerful biochemical method for the detection of proteins. Protein bands that were separated by size after PAGE are transferred and immobilized on a carrier membrane. The firm binding of the proteins to the membrane allows a subsequent protein detection by choosing from a variety of staining or immunological methods.
Wet-transfer Western Blot system
The FastGene® Western Blot System is an efficient device to perform the first Western Blot protein transfer step from gel to membrane via a wet electropohoretic transfer technique. The Western Blot Systems contains all the necessary components for reliable protein transfer and comes with detailed guidelines to successfully perform transfer and Western Blot analysis.
The system includes a cool pack, which is installed during the run. The cool pack absobs generated running heat, avoiding the possibility of power loss. Gel and membrane can be easily sandwiched in the blot connector, which is assembled via an easy-to-use lock mechanism.
Below you find an image of the general Western Blot setup.
Ordering information
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| PG08 | FastGene Western Blot System | 1 each | 853.00 |
Membranes for Western Blot
Polyvinylidene Fluoride (PVDF) membranes
PVDF (polyvinylidene difluoride) membranes can be used for amino-terminal sequencing, amino acid analysis and various kind of immunoassays. They retain bound protein even in the presence of organic solvents or acidic/basic conditions. PVDF membranes show a better binding efficiency of proteins with presence of SDS in the transfer buffer. They must be soaked with 100% methanol before use.
Nitrocellulose membranes
Nitrocellulose membranes are widely used for western blotting and protein detection. Colorimetric protein staining can be accomplished by protein specific dyes such as amido black, coomassie blue, ponceau S or colloidal gold. Nitrocellulose membranes are also used in immunologial detection methods such as radioimmunoassays, chemiluminescenceimmunoassays, fluorescence-immunoassays and enzyme-linked immunoassays. Proteins can bind strongly and almost irreversibly to the membrane with 80- 100 µg/cm2.
Accessories
FastGene® Western Blot Components - PG09
Components for blotting system
included in PG08
- 1x blot frame with electrodes
- 2x blot connector
- 5x blot sponge
- 2x blot cool pack
FastGene® Gel/Blot Chamber Tank - PG05
Large gel electrophoresis buffer tank (1 chamber tank)
included in PG08
The FastGene® Western Blot System (PG08) uses the same FastGene® Chamber Tank (PG05) and FastGene® Gel/Blot Chamber Lid as the FastGene® PAGE Protein System (PG01).
The FastGene® PAGE Protein System (PG01) can easiliy be upgraded for Western Blotting by ordering the FastGene® Western Blot Components (PG09).