PKmem dyes were developed by the lab of Zhixing Chen at Peking University. They label the plasma membrane in live cells with very high specificity. The unique and unmatched feature of PKmem dyes is their extremely low phototoxicity, due to the presence of the intramolecular triplet quencher cyclooctatetraene (COT) group. PKmem probes allow long term live cell imaging of the plasma membrane without damaging the cells and they do not require any genetic manipulation, transfection or overexpression of fluorescent proteins.
Key features
- Fast staining within 5-15 minutes
- Massively reduced phototoxicity compared with other membrane probes
- Highly specific staining of the plasma membrane
- SIM and STED compatible
- Compatible with PFA fixation and immunostaining
