Start CRISPRing the easy way
Are you planning to start an experiment using CRISPR-Cas9 genome editing? Here we present a range of useful CRISPR-Cas9 products from our supplier Integrated DNA Technologies (IDT). This overview contains all reagents necessary for a complete genome editing experiment and helps you assemble your personal CRISPR-Cas9 starter kit.
Use of the CRISPR and associated Cas9 enzymes for genome editing has been a major technological breakthrough. IDT's Alt-R™ CRISPR-Cas9 System is an optimized genome editing solution that outperforms other CRISPR approaches for producing on-target, double-stranded DNA breaks:
- Controlled delivery, high targeting efficiency, reduced off-target effects
- Chemically modified guide RNAs for improved stability
- Industry-leading HiFi Cas9 enzymes with unsurpassed specificity
- Precise HDR knock-ins with Alt-R HDR Donor Oligos or Alt-R HDR Donor Blocks
- HDR enhancer to increase the rate of homology-directed repair
- Protect your cells from toxicity or innate immune response activation
Select from the list of products below and order them on IDT's website. For additional information about genome editing using CRISPR-Cas, download IDT's CRISPR basics handbook or visit our overview page.
CRISPR essentials
Item | Catalog number | Amount | Description |
|---|---|---|---|
| Guide RNA | |||
| Alt-R™ CRISPR-Cas9 crRNA | Custom | 2 nmol | Targets a locus adjacent to a PAM site in your gene of interest. To be used with tracrRNA to form a functional gRNA duplex. Contains chemical modifications to protect from degradation by cellular RNases. |
| Alt-R™ CRISPR-Cas9 tracrRNA | 1072532 | 5 nmol | Universal 67mer tracrRNA that contains proprietary chemical modifications conferring increased nuclease resistance. Hybridizes to crRNA to activate the Cas9 enzyme. |
| OR | |||
| Alt-R™ CRISPR-Cas9 sgRNA | Custom | 2 nmol | Single RNA molecules comprised of both crRNA and tracrRNA sequences. Contains chemical modifications for a high level of functional stability. |
| Cas9 nuclease | |||
| Alt-R™ S.p. Cas9 Nuclease V3 | 1081058 | 100 µg | Recombinant S. pyogenes Cas9 nuclease, purified from an E. coli strain expressing the nuclease. |
| OR | |||
| Alt-R™ S.p. HiFi Cas9 Nuclease V3 | 1081060 | 100 µg | Cas9 variant that effectively reduces off-target effects and preserves high on-target activity. |
For experiments using homology-directed repair (HDR)
Item | Catalog number | Amount | Description |
|---|---|---|---|
| Alt-R™ HDR Donor Oligos | Custom | 2 nmol | Single-stranded DNA oligos for HDR. Up to 200 bases long and ideal for introducing point mutations or short insertions. |
| Alt-R™ HDR Donor Blocks | Custom | 3 µg | Modified linear dsDNA fragments for HDR, available from 201 to 3000 bases in length. |
| Alt-R™ HDR Enhancer V2 | 10007910 | 30 µL | Small molecule compound that has an ability to increase the rate of HDR. |
You might also need
Item | Catalog number | Amount | Description |
|---|---|---|---|
| Alt-R™ Cas9 Electroporation Enhancer | 1075915 | 2 nmol | Purified carrier DNA that improves delivery of Cas9 ribonucleoprotein by electroporation. |
| Alt-R™ CRISPR-Cas9 Control Kit | 2 nmol | Separate kits for human, mouse, or rat. Includes tracrRNA, HPRT positive control crRNA, negative control crRNA#1, HPRT Primer Mix, and Nuclease-Free Duplex Buffer. | |
| Alt-R™ Genome Editing Detection Kit | 1075931 | 25 rxns | Each kit contains T7EI endonuclease, T7EI reaction buffer, and T7EI assay controls. |
| IDTE pH 7.5 (1X TE Solution) | 11-01-02-02 | 10 x 2 ml | IDTE (10 mM Tris, 0.1 mM EDTA) is IDT's recommended solution for resuspending and storing single-stranded DNA and RNA oligos. It has been shown to offer the most stability for the longest duration when compared to oligos stored dry or in water. |
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For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.