Peroxi_SPY probes are novel and unique fluorescent live cell peroxisome probes. They specifically label peroxisomes in living cells as quickly as 15 min after addition to the medium. Peroxi_SPY probes are bright, non toxic, cell permeable and highly specific. They do not require transfection of reporter constructs and work by simply adding the probe to the cells and performing imaging a few minutes later.
Peroxi_SPY probes are available in two wavelengths: Peroxi_SPY555 and Peroxi_SPY650.
Key facts
- The first small molecule probe to specifically stain peroxisomes
- Non-toxic, ideal for live imaging
- Cell permeable
- Rapid staining (15 min. post addition)
Peroxi_SPY555 (SC207)
| Absorption maximum | 555 nm |
| Emission maximum | 580 nm |
| Fluorescence lifetime | 2.4 ns |
| STED depletion wavelength | 660 nm or 775 nm |
| Works with fixed cells? | No |
Peroxi_SPY650 (SC507)
| Absorption maximum | 652 nm |
| Emission maximum | 674 nm |
| Fluorescence lifetime | 3 ns |
| STED depletion wavelength | 775 nm |
| Works with fixed cells? | No |
Staining of zebrafish embryos using Peroxi_SPY probes
References
Daria Korotkova et Al. “Fluorescent fatty acid conjugates for live cell imaging of peroxisomes”, Nat Commun 15, 4314 (2024)