Midori Green - The safe DNA stain
A long period of time, ethidium bromide has been used as standard dye for staining of nucleic acids. Although today EtBr is considered to be mutagenic and carcinogenic, is has persisted in some laboratories as a DNA-intercalating substance, because it is supposed to be reliable and sensitive.
With our Midori Green dye product family, we offer green fluorescent stain for the visualization of DNA and RNA in agarose gels which is ultra-sensitive and safe (no risk for the scientist and no damage of the DNA). Midori Green is flexible and affordable.
- Replaces EtBr in all staining applications
- No need to change protocols - add to gels or use as loading buffer
- Compatible with UV boxes and Blue/Green LED technology
The safe alternative to Ethidium Bromide (EtBr)
Midori Green was developed to offer a non-carcinogenic and less mutagenic dye for DNA electrophoresis. In addition, Midori Green displays a high sensitivity for small fragments as well as a superior signal to noise ratio. The Midori Green products delivers even better DNA/RNA signals than ethidium bromide. However, this innovative DNA stain is non-carcinogenic, non-mutagenic and non-toxic and therefore not harmful for your health. Independent laboratories confirmed its safety: Both the mutagenicity test (ames test) and the cytotoxicity test were negative.
Midori Green is the only safe DNA stain on the market with a complete safety profile. Ask your supplier if your current "safe" DNA stain has passed these tests:
- Ames test
- Cytotoxicity
- Cell membrane permeability
- Hazardous waste screening
- Latex glove penetration test
For more information, read the safety reports of the products.
The products - get your "Greens" and keep yourself and your DNA healthy!
| Midori Green Easy - NEW! | Midori Green Xtra | Midori Green Direct | Midori Green Advance |
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| Read more below | Read more below | Read more below | Read more below |
Compatibility
Midori Green Easy - NEW!
Excellent signal quality
We developed MIDORIGreen Easy to have the same excellent signal quality and low background as MIDORIGreen Xtra. It is also unbeatable with visible excitation light and shows the best results with our Blue/Green LED technology. The bands show outstanding sharpness and sensitivity. MIDORIGreen Easy was designed to make the switch from SYBR® Safe as convenient as possible for you and get your DNA signals to the next level. If you are convinced by the quality of our MIDORIGreen Easy you can also try the more concentrated MIDORIGreen Xtra and adapt your staining protocol accordingly.
Easy switch from SYBR® Safe
MIDORIGreen Easy enables an effortless switch from using SYBR® Safe to stain agarose gels. MIDORIGreen Easy has exactly the same dye concentration (10,000 x) and tube volume (0.4 mL) as SYBR® Safe (sufficient for 4 L agarose) – for less than half the price.
The performance of MIDORIGreen Easy and SYBR Safe is the same, apart from the fact that heating of the DNA stain is recommended for SYBR Safe, whereas MIDORIGreen Easy should be added to the agarose after heating.
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| MG12 | Midori Green Easy (1 ml) | 1 ml | 81.00 |
Midori Green Xtra
Ultra-sensitive for LED illumination
Midori Green Xtra is a new highly sensitive green fluorescent stain for a safe visualization of DNA and RNA in agarose gels. This DNA stain is a safe and better alternative to the traditional nucleic acid stain ethidium bromide (EtBr). Remarkably, agarose gels stained with Midori Green Xtra have a very low background fluorescence, which makes the identification of low amounts of DNA very easy. Be one of the first and test Midori Green Xtra for a safe detection of DNA with an unbeatable sensitivity.
Ultra-sensitive DNA signals and low background – Just a perfect signal
Midori Green Xtra is optimized for Blue/Green and Blue LED light, leading to unbeatable fluorescence signals of DNA and RNA in agarose gels. In addition, UV-light is also suitable for the detection of nucleic acid, but less efficient than non-damaging visible light. Remarkably, Midori Green Xtra did not stain the agarose gel, leading to an excellent signal to noise ratio.
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| MG10 | Midori Green Xtra (1 ml) | 1 ml | 144.00 |
Midori Green Direct
Get the best signal to noise ratio
Midori Green Direct is just added to your samples. The loading dye is already included. You do not need to add any other loading dye to both gel matrix and running buffers. The direct staining of the DNA rather than the gel eliminates the background staining providing a perfect signal (Fig. 1). Midori Green Direct was developed to work with Blue Light LED illuminators, but you can also use it with a regular UV transilluminator.
Better Results for Downstream Application
The isolation of DNA from agarose gels enables downstream applications. It is well known that many dyes, such as ethidium bromide or even SYBR™ Green are strong enzyme inhibitors due to their intercalating properties. Midori Green dyes bind to the DNA backbone. This results in a much higher efficiency for downstream applications, i.e. cloning (Fig. 2), seqeuncing, PCR, etc.
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| MG06 | Midori Green Direct (1 ml) | 1 ml | 120.00 |
Midori Green Advance
Midori Green Advance is a non-carcinogenic dye. Optimised for a brighter signal when excited by UV-light or Blue/Green light. It has the advantages, such as being non-carcinogenic and having an excellent signal-to-noise ratio. It can be used just like EtBr by adding it directly to agarose and is compatible with regular UV tables as well as with new Blue LED or Blue/Green LED tables and instruments.
Midori Green Advance shows a very high sensitivity even for small DNA fragments. The dilution factor of Midori Green Advance can be as high as 1:25000. Hence, 4-6 μL are enough for the staining of a 100 mL agarose gel, resulting in ~17 to 25 liters of stained agarose gels.
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| MG04 | Midori Green Advance (1 ml) | 1 ml | 123.00 |
Midori Green helps you clone your DNA
When used in combination with Blue/Green LED transilluminators, Midori Green results in significantly less damage when cutting DNA from agarose gels and hence dramatically improves cloning efficiency.