Webinar: How to optimize CRISPR genome editing in primary T cells
This webinar, IDT will present optimized methods for CRISPR editing in primary T cells including optimized cell culture and delivery parameters for high-efficiency editing. Additionally, we will discuss methods for improving knock-in rates with both short and long inserts through optimized design of HDR donors and the use of HDR enhancing reagents.
Online
Genome editing in primary T lymphocytes has unique challenges that make generating correct clones a significant bottleneck in the progression of research. This webinar will present optimized methods for CRISPR editing in primary T cells including optimized cell culture and delivery parameters for high-efficiency editing. Additionally, we will discuss methods for improving knock-in rates with both short and long inserts through optimized design of HDR donors and the use of HDR enhancing reagents.
In this webinar, you will learn how to:
- Define different double-strand break repair pathways and utilize them for CRISPR editing
- Carry out your own CRISPR editing experiments in primary T cells
- Achieve high-efficiency knock-in rates in primary T cells with optimized methods and reagents