BioToolomics is a life science company specialising in the design, development and manufacturing of innovative process chromatography media and columns for the bioscience and bioprocessing industry. The company was established in February 2006 and is headquartered in the North East of England.
Discover some of the top products below.
ViralPolish
BioToolomics developed ViralPolish® chromatography media specifically for virus purification; Comprising dual layer agarose beads with an inert external shell with tightly controlled pore size and internal polyfunctional ligands for rapid high capacity binding of impurities.
This media is ideal for rapid clean-up, either as a first step or as a second step, to purify virus particles. Large particles, such as viruses, are excluded from the beads. The beads can be packed in a column where the virus will pass through the column bed and collected in the flow through fraction, whilst impurities will be captured within the beads. This affords a very gentle purification process resulting in efficient clean up and high recovery of active virus particles. The beads can also be used in batch mode; when added to a flask of virus containing media the beads will selectively allow small molecules to enter and be retained, whilst the virus is excluded and remains in the external media.
A typical purification strategy for virus would be to use ViralPolish® as an initial step, to clean up the sample, prior to binding and elution, typically from an anion exchange media designed for virus purification such as SepFast Supor Q. Alternatively, very dilute samples can be purified by first passing through a SepFast Supor Q anion exchange column to concentrate the sample followed by passing through ViralPolish column to remove residual impurities.
ViralPolish® is available in three different outer shell pore sizes, and two different impurity binding chemistries.
Protein A & Alkaline-stable Protein A SepFast Range
Protein A SepFast is a family of affinity chromatography media for the purification of immunoglobulins. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
- Protein A SepFast HighRes: 20 – 50 µm, good for small column run at shorter residence time (i.e. higher flowrate);
- Protein A SepFast HighRes Endure: 20 – 50 µm, more alkaline-stable than Protein A SepFast HighRes, can be cleaned with 0.5 M NaOH;
- Protein A SepFast: 50 – 150 µm, good for most applications from small scale to large scale purification requirements (i.e. low column pressure);
- Protein A SepFast Endure: 50 – 150 µm, more alkaline-stable than Protein A SepFast, can be cleaned with 0.5 M NaOH;
- Protein A SepFast Large Bead: 150 – 350 µm, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure);
- Protein A SepFast Large Bead Endure: 150 – 350 µm, more alkaline-stable than Protein A SepFast Large Bead, can be cleaned with 0.5 M NaOH;
Standard protein A molecule or more alkaline-stable protein A is immobilised to highly porous and highly cross-linked agarose base matrices. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The medium shows high mechanical rigidity, so it can be operated at moderate to high flow velocities with moderate pressure drop.
BioToolomics offers both loose media and pre-packed ready-to-use disposable columns for small scale sample preparation and large-scale bioprocessing use.
The range of Protein A SepFast media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.
Disposable Chromatography columns
Biotoolomics offers scalable chromatography columns for fast medium screening, process development and scaling-up applications, or general laboratory purifications. The empty column sizes range from 1ml to 40ml, and packed column sizes range from 1ml to 10 litres.
Our range of chromatography columns has the following key features and advantages:
- Fully scalable column design for bioprocessing applications
- Low cost
- Resin compressibility taken into account
- Minimum dead volume for high resolution applications
- Proven flow distribution mechanism
- Disposable
- Easy to pack
- Tight packing of any type of chromatography media (>20 µm)
- Features standard connections, which are compatible with most common chromatography instruments such as AKTA etc.
- Easy to store
- Long life time
After the chosen resin is packed in the column, the column can be readily stored and re-used for many times.
Documents
| Filename | Download |
|---|---|
| Biotoolomics-Gel_Filtration_Media.pdf |  |
| BioToolomics-Leaflet_activated_resins.pdf | |
| BioToolomics-Leaflet_column_packing_service.pdf | |
| BioToolomics-Leaflet_for_NCAP_antibody_purification_resins.pdf | |
| BioToolomics-Leaflet_for_Virus_Vaccine_Purifications.pdf | |
| BioToolomics-Leaflet_IEX_resins.pdf | |
| BioToolomics-Leaflet_Magnetic_resins.pdf | |
| BioToolomics-Leaflet_Protein-A_resins.pdf | |
| BioToolomics_-_Leaflet_for_pre-packed_laboratory_columns.pdf | |
