Immunophenotyping using CD antibodies

Cluster of differentiation (CD) are cell surface molecules expressed on the cells of the immune system, which play key roles in immune cell-cell communication, sensing the microenvironment and in adaptive immunity. During lymphocyte maturation, immune cells will express complex variations of CD antigens on its cell surface, some of which are lost, while others are acquired at various stages either by interactions with antigen presenting cells (APC) or with other cells of the immune system. In flow cytometric immunophenotyping, monoclonal antibodies targeting CD antigens are extensively used for the identification and characterization of leukocyte populations, subpopulations and differentiation stages. AAT Bioquest features purified anti-human CD antibodies and fluorescently labeled CD antibody conjugates applicable for a wide range of research disciplines including, flow cytometry, immunohistochemistry and immunoblotting applications.

Anti CD antibodies

AAT Bioquest offers a comprehensive selection of purified CD antibodies and fluorescently labeled CD antibody conjugates for multicolor flow cytometry, immunohistochemistry and functional assays.

Antibody	Clone	Unit size	Cat. no.
Anti-human CD1 Antibody	HI149	100 µg	10010000
Anti-human CD2 Antibody	HIT11	100 µg	10020000
Anti-human CD2 Antibody	RPA-2.10	100 µg	10021000
Anti-human CD3 Antibody	HIT3a	100 µg	10030000
Anti-human CD3 Antibody	HIT3b	100 µg	10031000
Anti-human CD3 Antibody	OKT-3	100 µg	10034000
Anti-human CD3 Antibody	SK7	100 µg	10033000
Anti-human CD3 Antibody	UCHT1	100 µg	10032000
Anti-human CD4 Antibody	RPA-T4	100 µg	10041000
Anti-human CD4 Antibody	HIT4a	100 µg	10040000
Anti-human CD4 Antibody	OKT-4	100 µg	10043000
Anti-human CD4 Antibody	SK3	100 µg	10042000
Anti-human CD5 Antibody	HISM2	100 µg	10050000
Anti-human CD5 Antibody	L17F12	100 µg	10051000
Anti-human CD5 Antibody	UCHT2	100 µg	10052000
Anti-human CD6 Antibody	HI210	100 µg	10060000
Anti-human CD7 Antibody	HIT7	100 µg	10070000
Anti-human CD8 Antibody	SK1	100 µg	10081000
Anti-human CD8 Antibody	HIT8a	100 µg	10080000
Anti-human CD8 Antibody	OKT-8	100 µg	10082000
Anti-human CD9 Antibody	HI9a	100 µg	10090000
Anti-human CD10 Antibody	HI10a	100 µg	10100000
Anti-human CD11a Antibody	HI111	100 µg	10110000
Anti-human CD11b Antibody	HI11b	100 µg	10111000
Anti-human CD11b Antibody	ICRF44	100 µg	10112000
Anti-human CD11c Antibody	3.9	100 µg	10113000
Anti-human CD13 Antibody	WM15	100 µg	10130000
Anti-human CD14 Antibody	61D3	100 µg	10141000
Anti-human CD14 Antibody	HI221	100 µg	10140000
Anti-human CD15 Antibody	HI98	100 µg	10150000
Anti-human CD18 Antibody	HI18a	100 µg	10180000
Anti-human CD19 Antibody	HI19a	100 µg	10190000
Anti-human CD19 Antibody	HIB19	100 µg	10192000
Anti-human CD19 Antibody	SJ25C1	100 µg	10191000
Anti-human CD20 Antibody	HI20a	100 µg	10201000
Anti-human CD20 Antibody	HI47	100 µg	10200000
Anti-human CD21 Antibody	HI21a	100 µg	10210000
Anti-human CD22 Antibody	HIB22	100 µg	10220000
Anti-human CD24 Antibody	HI45	100 µg	10240000
Anti-human CD25 Antibody	HI25a	100 µg	10250000
Anti-human CD28 Antibody	CD28.2	100 µg	10280000
Anti-human CD29 Antibody	HI29a	100 µg	10290000
Anti-human CD33 Antibody	HI33a	100 µg	10330000
Anti-human CD33 Antibody	HIM3-4	100 µg	10331000
Anti-human CD34 Antibody	4H11	100 µg	10340000
Anti-human CD38 Antibody	HI157	100 µg	10381000
Anti-human CD38 Antibody	HIT2	100 µg	10380000
Anti-human CD40 Antibody	HI40a	100 µg	10400000
Anti-human CD44 Antibody	HERMES-1	100 µg	10443000
Anti-human CD44 Antibody	HI44a	100 µg	10440000
Anti-human/mouse CD44 Antibody	IM7	100 µg	10441000
Anti-human CD45 Antibody	2D1	100 µg	10454000
Anti-human CD45 Antibody	HI100	100 µg	10455000
Anti-human CD45 Antibody	HI151	100 µg	10452000
Anti-human CD45 Antibody	HI185	100 µg	10453000
Anti-human CD45 Antibody	HI30	100 µg	10450000
Anti-human CD45 Antibody	HI73	100 µg	10451000
Anti-human CD45 Antibody	UCHL1	100 µg	10456000

iFluor™ dyes labeled to CD antibodies

The following table outlines the fluorescence properties of available iFluor™ dye labeled anti-human CD antibodies for use in flow cytometry (FACS) and fluorescence imaging applications. Conjugates made with iFluor™ dyes exhibit superior brightness and photostability, outperforming Alexa Fluor® conjugates and other spectrally similar conjugates. For additional information on iFluor™ dye-labeled CD antibodies and availability of other clones click on any label in the table below.

Label	Ex (nm)	Em (nm)	ε¹	Φ²	CF 260 nm³	CF 280 nm?
iFluor™ 350	344	448	20,000	0.95	0.83	0.23
iFluor™ 405	402	425	37,000	0.91	0.48	0.77
iFluor™ 430	433	495	40,000	0.78	0.68	0.3
iFluor™ 450	451	502	40,000	0.82	0.45	0.27
iFluor™ 488	491	516	75,000	0.9	0.21	0.11
iFluor™ 514	527	554	80,000	0.83	0.25	0.11
iFluor™ 532	543	563	90,000	0.68	0.26	0.16
iFluor™ 546	541	557	100,000	0.67	0.25	0.15
iFluor™ 555	556	569	100,000	0.64	0.23	0.14
iFluor™ 560	559	571	130,000	0.57	0.048	0.069
iFluor™ 568	568	587	100,000	0.57	0.34	0.15
iFluor™ 594	587	603	180,000	0.53	0.05	0.04
iFluor™ 610	609	627	110,000	0.85	0.32	0.49
iFluor™ 633	638	652	250,000	0.29	0.062	0.044
iFluor™ 647	654	669	250,000	0.25	0.03	0.03
iFluor™ 660	660	677	250,000	0.26	0.07	0.08
iFluor™ 670	669	682	250,000	0.63	0.019	0.024
iFluor™ 680	683	700	220,000	0.23	0.097	0.094
iFluor™ 700	690	713	220,000	0.23	0.09	0.04
iFluor™ 710	712	736	190,000	0.14	0.12	0.07
iFluor™ 750	759	777	275,000	0.12	0.044	0.039
iFluor™ A7	759	780	275,000	0.1	0.03	0.03
iFluor™ 790	786	811	250,000	0.13	0.1	0.09
iFluor™ 800	801	820	250,000	0.11	0.03	0.08
iFluor™ 810	811	822	250,000	0.05	0.09	0.15
iFluor™ 820	820	849	250,000	N/A	0.11	0.16
iFluor™ 840	836	876	200,000	0.02	0.2	0.09
iFluor™ 860	852	877	250,000	N/A	0.1	0.14

Note

ε = molar extinction coefficient at their maximum absorption wavelength (Units = cm^-1M^-1).
Φ = fluorescence quantum yield in aqueous buffer (pH 7.2).
CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm (for oligo and nucleic acid labeling).
CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).

mFluor™ dyes labeled to CD antibodies

The following table outlines the fluorescence properties of available mFluor™ dye labeled anti-human CD antibodies for use in flow cytometry (FACS). Each mFluor™ dyes is optimally excited by one of the major laser lines commonly equipped in flow cytometers, such as the 405 nm, 488 nm, 532 nm, 561 nm or 633 nm laser lines. For additional information on mFluor™ dye-labeled CD antibodies and availability of other clones click on any label in the table below.

Label	Ex (nm)	Em (nm)	ε¹	Φ²	CF 260 nm³	CF 280 nm?
mFluor™ UV375	354	388	35,000	0.94	0.099	0.138
mFluor™ Violet 450	406	445	25,000	0.92	0.338	0.078
mFluor™ UV460	362	456	15,000	0.86	0.35	0.134
mFluor™ Violet 500	426	497	35,000	0.81	0.769	0.365
mFluor™ Violet 510	409	504	30,000	0.86	0.464	0.366
mFluor™ Violet 540	400	532	15,000	0.64	1.392	0.529
mFluor™ Blue 570	552	564	120,000	0.08	0.228	0.179
mFluor™ Green 620	521	617	50,000	0.06	0.895	0.569
mFluor™ Yellow 630	546	625	110,000	0.01	0.283	0.413
mFluor™ Red 700	657	694	250,000	0.029	0.135	0.127
mFluor™ Red 780	631	768	70,000	0.034	0.101	0.116

Note

ε = molar extinction coefficient at their maximum absorption wavelength (Units = cm^-1M^-1).
Φ = fluorescence quantum yield in aqueous buffer (pH 7.2).
CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm (for oligo and nucleic acid labeling).
CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).

Alexa Fluor® dyes labeled to CD antibodies

The following table outlines the fluorescence properties of available Alexa Fluor® dye labeled anti-human CD antibodies for use in flow cytometry (FACS) and fluorescence imaging applications. For additional information on Alexa Fluor® dye-labeled CD antibodies and availability of other clones click on any label in the table below.

Label	Ex (nm)	Em (nm)	ε¹	Φ²	CF 280 nm³
Alexa Fluor® 350	343	441	19,000	N/D	0.19
Alexa Fluor® 488	499	520	73,000	0.92	0.11
Alexa Fluor® 555	553	568	155,000	0.1	0.08
Alexa Fluor® 594	590	618	92,000	0.66	0.56
Alexa Fluor® 647	650	671	270,000	0.33	0.03
Alexa Fluor® 680	681	704	183,000	0.36	0.05
Alexa Fluor® 700	696	719	205,000	0.25	0.07
Alexa Fluor® 750	752	776	290,000	0.12	0.04

Note

? = molar extinction coefficient at their maximum absorption wavelength (Units = cm^-1M^-1).
? = fluorescence quantum yield in aqueous buffer (pH 7.2).
CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).

Classic dyes labeled to CD antibodies

The following table outlines the fluorescence properties of available classic dye labeled anti-human CD antibodies for use in flow cytometry (FACS) and fluorescence imaging applications. For additional information on classic dye-labeled CD antibodies and availability of other clones click on any label in the table below.

Label	Ex (nm)	Em (nm)	ε¹	Φ²	CF 280 nm³
Pacific Blue™	404	455	46,000	0.78	0.2
FITC	491	516	73,000	0.79	0.254
Pacific Orange™	400	551	25,000	N/D	0.6
TRITC	544	570	85,000	0.1	0.34

Note

? = molar extinction coefficient at their maximum absorption wavelength (Units = cm^-1M^-1).
? = fluorescence quantum yield in aqueous buffer (pH 7.2).
CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).

PE, APC, PerCP and tandem dyes labeled to CD antibodies

The following table outlines the fluorescence properties of available phcoerythrin (PE), allophycocyanin (APC), PerCP and tandem dye labeled anti-human CD antibodies for use in flow cytometry (FACS). Phycobiliproteins are uncharacteristically bright due to their high molar extinction coefficients and quantum yields, an enviable quality when imaging low-abundance targets. However, since phycobiliprotiens photobleach rapidly, they are not recommended for microscopy. For additional information on phycobiliprotein-labeled CD antibodies and availability of other clones click on any label in the table below.

Label	Ex (nm)	Em (nm)	ε¹	Laser Line (nm)
PE	566	574	1,960,000	488 nm, 532 nm, 561-568 nm
PE-Texas Red®	567	615	1,960,000	488 nm, 532 nm, 561-568 nm
PE-Alexa Fluor® 610	567	627	1,960,000	488 nm, 532 nm, 561-568 nm
PE-Cy5®	565	666	1,960,000	488 nm, 532 nm, 561-568 nm
PE-iFluor™ 647	569	666	1,960,000	488 nm, 532 nm, 561-568 nm
PE-Alexa Fluor® 700	566	721	1,960,000	488 nm, 532 nm, 561-568 nm
PE-Cy7®	566	778	1,960,000	488 nm, 532 nm, 561-568 nm
PE-iFluor™ 750	566	778	1,960,000	488 nm, 532 nm, 561-568 nm
APC	651	660	700,000	633-647 nm
APC-iFluor™ 700	651	710	700,000	633-647 nm
APC-Cy7®	651	779	700,000	633-647 nm
APC-Alexa Fluor® 750	651	785	700,000	633-647 nm
APC-iFluor™ 750	651	791	700,000	633-647 nm
PerCP	477	678	350,000	488 nm
PerCP-Cy5.5	489	679	350,000	488 nm

Note

? = molar extinction coefficient at their maximum absorption wavelength (Units = cm^-1M^-1).

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