Introduction
Monkeypox has been termed a global health emergency.
Monkeypox is a virus from the orthopox family of viruses, a family most often associated with the more severe smallpox virus (variola), or the origination of vaccinations from the cowpox virus. Understanding how the virus is being spread, and the immune responses against the virus are of increasing importance in the research field. LubioScience is proud to offer a variety of research tools and reagents to support the Monkeypox research community.
xGen™ Monkeypox Virus Amplicon Panel
xGen Amplicon Technology uses overlapping primer sets to provide comprehensive coverage of positions 6760–190,905 (ITRs not included) from as few as 300 monkeypox viral genome copies. Track the spread in a single-tube, ~2.5-hour workflow.
For researchers interested in tracking the evolution of the monkeypox virus genome, it can be difficult to get sufficient coverage. The xGen Monkeypox Virus Amplicon Panel provides a single-tube, two-step PCR amplification workflow with primer sets designed to create amplicons across the entire genome.*
The xGen Monkeypox Virus Amplicon Panel supports:
- Comprehensive coverage from positions 6760–190,905 (ITRs not included)
- Sequence data from viral titers as low as 300 viral genome copies
- Single-tube workflow
- Viral-DNA-to-sequencer in ~2.5 hours
- Up to 1536 UDIs
- Super amplicon technology
xGen™ Monkeypox Virus Amplicon Panel
Monkeypox virus panel that utilizes multiple overlapping amplicons in a single tube, comprises a premixed target-specific primer pool.
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| 10015906 | xGen Monkeypox Virus Amplicon Panel 96rxn | 96 reactions | 1'085.00 |
xGen™ Amplicon Core Kit
Targeted amplicon library prep kit for contiguous coverage (overlapping amplicons), comprises multiplexed PCR, indexing PCR, and Normalase™ reagents (target-specific panel and indexing primers sold separately).
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| 10009827 | xGen Amplicon Core 96rxn | 96 reactions | 3'496.00 |
xGen™ Amplicon UDI Primers
Premixed Normalase unique dual index primer pairs for sample indexing during library amplification and conditioning for Normalase enzymology, 1536 ten base index pairs dispensed into 16 96-well plates, Reagent R7 NOT provided, instead uses reagent supplied in the xGen amplicon core kit.
Brief Introduction of MPXV Protein
Monkeypox Virus (MPXV) A35 Protein：
MPXV A35R protein is a homolog of Vaccinia virus A33R protein.
A33R protein plays a vital role in efficient cell-to-cell spread of viral particles. It is a potential target for diagnostic tests, vaccine/drug development.
Monkeypox Virus (MPXV) A29 Protein：
The structure and function of monkeypox virus A29 protein is less studied, but it is a homolog of Vaccinia virus A27 protein.
Multiple functions of A27 protein: 1) Bind heparin on the cell surface to mediate cell fusion; 2) Required for IMV trafficking on microtubules and IEV formation; 3) Anchor for packaging A26 protein into mature viral particles; 4) Key target for neutralizing antibody.
Monkeypox Virus (MPXV) H3L Protein：
MPXV H3L protein is a homolog of Vaccinia virus H3L protein.
H3L protein is envelope protein that binds to heparan sulfate on the cell surface.It might provide virion attachment to host cell. It is a potential target for vaccine or drug development.
Monkeypox Virus (MPXV) I1L Protein：
MPXV I1L protein is Expressed in the late phase of the viral replicative cycle. It is a DNA-binding protein which binds to the hairpin form of the viral telomeric sequence.
Monkeypox Virus (MPXV) L1R Protein：
MPXV L1R protein is a homolog of Vaccinia virus J1R protein. J1R protein plays a vital role in virus growth, plaque formation, and virion morphogenesis.
Recombinant Monkeypox Virus Antigens
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| 40886-V08H-100UG | Monkeypox Virus (MPXV) Protein A35 (His Tag) | 100 ug | 599.00 |
| 40893-V08H1-100UG | Monkeypox Virus (MPXV) Envelope protein H3 (His Tag) | 100 ug | 735.00 |
| 40891-V08E-100UG | Monkeypox Virus (MPXV) Protein A29 (His Tag) | 100 ug | 735.00 |
| 40889-V07E-100UG | Monkeypox Virus (MPXV) L1R Protein (His Tag) | 100 ug | 599.00 |
| 40896-V07E-100UG | Vaccinia virus (VACV) (strain Copenhagen) A33R Protein (His Tag) | 100 ug | 735.00 |
| 40903-V07H-100UG | Vaccinia virus (VACV) (strain Copenhagen) L1R Protein (His Tag) | 100 ug | 735.00 |
Monkeypox Virus Antibodies
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| 40891-M0036-100UL | Monkeypox Virus (MPXV) A29 Antibody, Mouse MAb | 100 ul | 357.00 |
| 40891-M0031-100UL | Monkeypox Virus (MPXV) A29 Antibody, Mouse MAb | 100 ul | 357.00 |
| 40889-T62-100UL | Monkeypox Virus (MPXV) L1R Antibody, Rabbit PAb, Antigen Affinity Purified | 100 ul | 357.00 |
| AB02217-1.1 | Anti Monkeypox Virus [VACV-5C7], IgG1 | 200 ug | 645.00 |