Summary
Cryopreservation reagents have been developed primarily for freezing isolated cells, with limited research into their efficacy for freezing tissues. In this study, mouse spleens were frozen at -80°C for 10 days using commercially available cryopreservation reagents and the thawed cells were analysed using a flow cytometer. Bambanker proved to be the optimal choice, demonstrating superior cell viability and integrity.
Experimental setup
Spleens of BALB/c mice were collected and prepared for cryostorage using either Bambanker or a competitor cell freezing agent (Cellbanker 2, Stem-Cellbanker GMP grade). After storage at -80°C for 10 days the spleen samples were thawed and subjected to single cell dissociation. Single cells were stained using surface markers and a live/dead stain and analyzed using either a cell counter or FACS.
Brief results
Cell viability analysis of isolated spleen cells after cryostorage showed that all three cell freezing media are suitable for whole spleen cryopreservation. Bambanker showed slightly higher cell viability scores than competitor products. In summary, Bambanker cell freezing medium is suitable for cryopreservation of whole mouse spleen, with acceptable recovery rates after freezing and thawing. This application note indicates that cryopreservation of whole mouse organs is feasible using a suitable cryopreservation agent, such as Bambanker.
Download the full application note
| Filename | Download |
|---|---|
| AN-BB0-Bambanker-tissue-freezing-competitor-analysis.pdf |  |