Description
Photo-cleavable (PC) modifications contain a photolabile functional group that is cleavable by UV light of specific wavelength (300–350 nm). The PC Spacer is a 10-atom linker arm that can only be cleaved when exposed to UV light within the appropriate spectral range. The resulting oligo will have a 5’ phosphate group that is available for subsequent ligase reactions.
PC Spacers can be placed between DNA bases or between an oligo and a terminal modification such as a fluorophore. IDT also offers 5’ PC Biotin (/5PCBio/), which allows for post-binding release and the design of controlled capture-and-release assays.
To order, select PC Spacer or PC Biotin from the 5’ Mods or Internal Mods tab on the oligo order page. Note that this modification requires HPLC purification.
![Integrated DNA Technologies, IDT](https://www.lubio.ch/assets/_processed_/8/1/csm_photo-cleavable-linker_ed8a14f005.png)
Quick Facts
Availability: DNA or RNA
Scales: 100 nmol to large scale
Purification: HPLC required
Symbol for ordering: 5’ mod: /SSpPC/, internal mod: /iSpPC/