Our colleagues at Pivotal Scientific investigate the different methods used to generate antibodies. While the standard method of generating antibodies in animals has existed for decades, it has its drawback and hence there is a growing interest in generating synthetic affinity reagents. Read this blog article and learn more about non-antibody-based alternatives and recombinant or synthetic antibodies.
Read part 1 here.

Introduction

The classic method used to generate antibodies for research purposes is to immunize an animal with the protein of interest (or a part thereof), and allow the animal to elicit an immune response to generate antibodies that specifically bind to the immunised protein. The antibodies can then be purified from the serum or plasma. Standard protocols for this process have existed for many decades. Owing to their high specificity, such immune reagents are generally preferred over non-immune reagents.

However, antibodies generated this way have not always been used successfully or were not robust enough (1, 2) and the involvement of animals means variations in antibody performance from one animal to the next are inherent (3). Certain types of molecules, and even certain proteins, are not fit to elicit an immune response, and therefore this classical way of generating specific reagents is not always feasible. Hence the interest in synthetic (immuno-like) reagents.

When antibodies are generated and produced without the involvement of animals, they are regarded as synthetic antibodies, or recombinant antibodies (4). In addition, non-antibody based alternatives have also emerged, such as several forms of scaffold proteins displaying diversified amino acid sequences with specific affinity binding properties, chemically stabilised nucleic acid oligomers called aptamers, and molecularly imprinted polymers (MIPs). Moreover, they all have in common, a binding site optimized for highly specific and strong interaction with a particular part of the target molecule, called the epitope. This 2-part series will discuss these different groups of reagents in depth.

This is part 2 on the synthetic immune reagents as alternatives to antibodies raised in animals. In this part we focus on scaffold proteins and on the aptamers. In part 1, we have focused on the recombinant antibodies and on Molecularly Imprinted Polymers (MIPs built around the analyte of choice).

Scaffold proteins

What are they?

Affinity binders based on scaffold proteins have been designed and used as alternatives to antibodies (5), including Abdurins, Affibodies, Affimers, Affitins, Anticalins, Bicyclic peptides, DARPins, Fynomers, Kunitz domains, and Monobodies. They all follow the same concept: a stable scaffold protein is used to display diversified amino acid sequences at exposed surface sites. The best affinity binders are selected using an appropriate display platform (phage, yeast or ribosome display) in the same way as for the selection of recombinant antibodies (see part 1). From this follows that also for this type of affinity binders, the quality of the screen depends on the quality of the library, and on the quality of the material to screen against. And, as discussed for recombinant antibodies, also a counter screen to address potential cross-reactivity should be involved.

The diverse range of non-antibody protein scaffolds provides opportunity to exploit native characteristics of the relevant target-binding domain and to produce tailored applications (5). The advantage of a tailored approach is that wildtype functions of each binding domain can be leveraged to overcome limitations of the traditional immunoglobulin antigen binding site, such as the modular construction of DARPins (6), convex binding surfaces of Affibodies (7), protease targeting in Kunitz domains (8), or the versatility of the FN3 domains in monomers to design context-sensitive drugs or biosensors (9).

Because of the small size and the low-cost of production, non-immunoglobulin scaffolds as alternatives to antibodies are being evaluated in clinical trials (10) and they are likely to enter the commercial tool affinity binder market some time in the future. As with antibodies, validation for specific purposes and batch quality control remain necessary.

Examples

Monobodies are based on a human Fibronectin (adhesive glycoprotein) scaffold: The type III domains (FN3) are folded in a similar way as the antigen binding site of an antibody (Fig1). After the tenth FN3 domain was validated as a scaffold for molecular recognition (11, 12), the loops in the domain were engineered to different lengths and in different amino acid diversities to seek high specific binding characteristics to desired targets. Although a serine/tyrosine binary code is sufficient to generate nanomolar affinity interactions in an antigen-binding fragment (13, 14), subsequent studies revealed that the full amino acid diversity is superior over the binary code to find picomolar to low nanomolar binders (15).

Synthetic immune reagents - Antibody domains binding loops

Figure 1 (copy of Fig 1B in Chandler and Buckle 2020, ref 18). Antibody domains use a set of three hypervariable binding loops (CDRs) to form a complementary region to a target binding site (left). Fibronectin type III (FN3) domains have a comparable set of analogous loops which can be engineered for similar binding function, as well as an expanded binding footprint in the side and ‘south’ loops (right).

Because monobodies have no cystein residues, they can readily be expressed in intracellular compartments with a reducing environment without losing integrity. In addition, the capability of monobodies as crystallization chaperones facilitates studies on protein structure, which in turn advances the understanding of biological processes and improved structure-guided design (16). When monomers are designed for therapeutic targets, they may be called AdnectinsTM (17). Other monomer variants used for potential therapeutics include intrabodies, E10-FN3, Centyrins, FN3Con and Tenascin-FN3 (18).

The Abdurin scaffold was developed to address the disadvantage of the shorter half-life for the smaller antibody-like scaffolds for in vivo applications. Abdurins are derived from the CH2 domain of human IgG. They are small (12.5kDa) and they are stable as monomers in the absence of glycosylation and of other IgG subunits (19). Its structure strongly resembles the antigen binding site of IgG (fig 2), thus its loops can be engineered for the generation of large libraries of binders to target molecules of interest. High affinity binders to human EphA2 have been generated and used to specifically target tumours in a xenograft model (20). However, initial attempts to use abdurins as drug conjugates for tumour therapy were not as successful as one would have hoped for (21).

Synthetic immune reagents - Structure of the CH2 antibody domain

Figure 2. Structure of the CH2 antibody domain (copy of figure 1 of Prabakaran et al 2008, ref 19).

Affibodies are derived from the three helical Z domain of Staphylococcus Protein A, which is involved in the binding of the IgG Fc domain (22), and they were originally designed by randomization of 13 solvent-accessible surface residues on two of the three helices within this Z domain (Fig 3) for the identification of specificity to Taq DNA polymerase, human insulin and ApoA1 (23). The Affibody molecule is very robust, and has generated useful biotechnological tools, such as the alkali-resistant active component in a kit for affinity purification of mAbs (MabSelect SuRe) or as part of a hot start PCR kit, where the Affibody molecule improves the fidelity of the DNA polymerase and by making the polymerase survive a larger number of heat cycles (Phusion Hot Start II DNA Polymerase) (24).

Radiolabeled affibody molecules can provide highly specific and sensitive imaging. A phase I/II clinical trial showed that 68Ga-labeled affibody molecules permit an accurate and specific measurement of HER2 expression in breast cancer metastases (25). Radiolabelled affibodies specific to EGFR improve imaging of EGFR-expressing tumours (26).

Synthetic immune reagents - Structure of an affibody scaffold

Figure 3. Structure of an affibody scaffold. Asterisks mark the segments where randomized amino acids are located. (Copy of Figure1 in Tolmachev and Orlova 2020, ref 25).

More than six different Affibody molecules have been combinatorially fused with antibodies to form functional multispecific proteins called AffiMabs (27, 28) with superior in vivo therapeutic activity in a xenograft tumor model when administering a bispecific molecule based on the EGFR-specific antibody cetuximab and a HER3-specific Affibody molecule (27). And engineered multivalent affibodies to HER3 enhanced the downregulation in multiple cancer cell types (29). A HER2-specific affibody was conjugated to a near-infrared silicon phthalocyanine dye, IRdye700DX (IR700), and applied to a xenograft HER2 cancer (BT474) in mice. Light-irradiation activates IR700 to kill the cells at the conjugate’s location, thus showing efficacy for this type of potential therapy (30). Affibodies against Epstein Bar virus (EBV) protein LMP2A were able to reduce viability of EBV-positive cell lines and of nasopharyngeal carcinoma (NPC)-derived cell lines (31).

The adhiron scaffold is a synthetic protein originally based on a phytocystatin consensus sequence.  Adhirons display remarkable thermal stability (Tm = 101°C) (32). The adhiron scaffold is related in structure to a previously reported scaffold engineered from human stefin A (33). Binding proteins derived from these two scaffolds are now referred to collectively as Affimer proteins, or Affimers.

Synthetic immune reagents - Ribbon diagram for Affimer

Figure 4. Ribbon diagrams of the crystal structures for Affimer (Adhiron) reagents. The unchanged loops in pink (A), specific to p300 (B) and to SUMO protein (C) with the variable regions in pink. (Copied from Tiede et al, 2017, ref 34).

Affimers have demonstrated to be good alternatives to antibodies as research reagents as demonstrated by the generation and successful use of affimers against 12 different targets, including a small organic molecule (34), and a recent report concluded that for both IL37B and proinsulin, affimers show higher specificity in purifying their target proteins from human plasma compared to monoclonal antibodies (35). They also appear superior for quantification in LC/MS (Liquid Chromatography before Mass Spectrometry) as demonstrated for serum RAGE when they detect 25% lower sRAGE levels than the validated antibody-based method (36). And they are an excellent tool as anti-idiotypic reagents for pharmacokinetic studies of therapeutic antibodies (37). Finally, the manufacturer of the affimers, Avacta, has developed a rapid test to SARS-CoV-2: avacta.com/results-of-initial-evaluation-of-sars-cov-2-lateral-flow-antigen-test-with-clinical-samples/

The affitin scaffold is commonly derived from Sac7d, a dsDNA-binding protein from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Sac7d is chemically and thermally stable and is resistant to extreme pH. Its molecular organization is quite simple, being small (66 residues) and monomeric, lacking a disulfide bridge, and possessing only one structural domain (Figure 5). The residues involved in DNA binding can be varied to make the scaffold bind other targets of interest (38). After showing that affitins are fit as a scaffold alternative to antibodies by selection of specific reagents through ribosome display librabries (39), and fit to use for affinity purification (40), they have been successfully used in diagnostics to identify bacterial species (41). Sac7d-based Affitins are good for detection-, capture-, and inhibition-based applications, such as one-step ELISA and Western blots, immunolocalization, biosensors, protein-chip array, affinity chromatography, magnetic fishing, enzymatic and intracellular inhibition, and imaging (42).

Synthetic immune reagents - Schematic representation of wild-type Sac7d

Figure 5. Schematic representation of wild-type Sac7d. Residues involved in DNA binding and that were substituted are shown in yellow. (copied Figure 1 from Mouratou et al, 2007, ref PMID: 17984049

In addition to Sac7D, affinitins can also be based on similar archaea proteins such as 63 residues Sso7D (43) and the 60 residues Aho7C (42), all benefitting from high thermostability and small size without cysteins (no sulphur bridges for secondary structures).

Anticalins are derived from proteins of the lipocalin family, a family of diverse proteins that normally serve for the storage or transport of hydrophobic small molecules, such as biliverdin IXγ, a butterfly chromophore, retinal, an important pigment for visualization, or pheromones. Despite low mutual sequence homology, lipocalins share a conserved barrel of eight antiparallel β-strands as their central folding motif. At one end of this structure, four loops connect each pair of β-strands and these loop regions are involved in the specific binding of their target molecule. Thus, although being composed of a single polypeptide chain and being much smaller, the lipocalins resemble immunoglobulins, owing their vast potential of antigen specificities to the six hypervariable loops on top of a rigid framework. Initial anticalins were developed from the Pieris brassicae (butterfly large white) anticalin BBP (Bilin-Binding Protein) by panning a phage library for specificity to fluorescein (44) and to digoxigenin (45).

Synthetic immune reagents - Lipocalin domain of BBP

Figure 6. The lipocalin domain of BBP bound to the butterfly chromophore biliverdin IXγ (PMID: 10051566).

Since then, anticalins have been developed specific to small molecules, peptides and proteins based on the human Lipocalin 2 (Lcn2/NGAL) scaffold (46 and references therein, 47) and on human Lipocalin 1 (Lcn1/TLC) scaffold (48). Because of the small size, stability and capacity to specifically bind small molecules, like hapten, these human scaffolds are excellent vectors for pharmaceutical interventions. Hence anticalins are investigated in preclinical and clinical stages for therapy against a variety of diseases (49). 

The butterfly BBP scaffold remains useful as a detector for non-human targets, example for herbicide quantification (50).

Natural bicyclic peptides are generated as a protective toxin by multiple organisms. Examples are moroidin produced by the seed of Celosia argentea (plumed cock’s comb) that inhibits eukaryotic cell division by interference in tubulin polymerization (51), and phalloidin and a-amanitin produced by mushrooms interfering in the depolymerization of actin filaments (52), and inhibiting RNA polymerase in rats, respectively (53).

For the development of new specific binders, either synthetic libraries are constructed, or libraries are generated through biological production by ribosomal synthesis, phage-based production or through the mRNA display method (54). Thus, these small and stable peptide-based reagents may serve as therapeutics, such as enzyme inhibitors (55, 56, 57), inhibitors of protein interactions (58, 59), receptor agonist/antagonists (60, 61). In addition, they can also be used for imaging (62) and diagnostics (63), or as antimicrobial agent (64).

DARPins (Designed Ankyrin Repeat Proteins) may be especially interesting due to their high stability and high production levels when expressed in bacteria, their potential multivalence, possibility of site-specific conjugations, and their potential picomolar affinities (65). They are a cost-effective solution for large-scale production (the costs of generating a new DARPin are similar to making a custom monoclonal antibody), for detection or interference inside targeted cells, and for multiplex applications. They have been successfully used for chimeric antigen receptor-T cells, for viral manipulations and for cytoplasmic markers. Like for many scaffolds, the research market is not yet served commercially, but only through academic collaborations. Because of the attractive profit margins, commercial use has focused on therapeutics (66).

Fynomers are binding proteins derived from the SH3 domain of human tyrosine kinase Fyn. These scaffold proteins are composed of two β-sheets and two exposed loops for binding to target proteins. The Fyn SH3 domain is a particularly attractive scaffold for the generation of binding proteins because the resulting Fynomer (67):

  • Is expressed in bacteria in soluble form in high amounts
  • Is monomeric and does not aggregate when stored in solution
  • Is very stable (Tm ~70°C)
  • Lacks cysteine residues
  • Is of human origin featuring an amino acid sequence completely conserved from mouse to man and, hence, is considered to be non-immunogenic.
  • Fynomer candidates can be panned from a phage display library
  • High-affinity Fynomers can be obtained after randomization of the RT- or n-src-loop, and subsequent affinity maturation selection

Fynomers can be fused to N-terminal and/or C-terminal ends of antibodies to generate bispecific therapeutics (FynomAbs). For example, the bispecific HER2/CD3 targeting FynomAbs redirect T cells to HER2-expressing tumour cells, causing tumour cell lysis in multiple cell lines, using only picomolar quantities. The activity was found to be highly specific, as no lysis of cells was observed in the absence of HER2 expression (68). FynomAb COVA322 was designed to simultaneously inhibit the activity of both TNF and IL-17A for treatment of rheumatoid arthritis (69). COVA322 is currently being evaluated in a phase II clinical trial (NCT02243787).

Kunitz domains are ~60-amino-acid peptides derived from the active motif of Kunitz-type protease inhibitors such as Aprotinin (bovine pancreatic trypsin inhibitor), Alzheimer’s amyloid Precursor Protein, and Tissue Factor Pathway Inhibitor. Most important for library generation, they possess loop regions that can be mutated without destabilizing the structural framework. Thus, phage display libraries can be panned for the desired binding capacity. These binders have been used primarily for clinical applications (70, 71).

Aptamers

Aptamers are oligonucleotides, such as ribonucleic acid (RNA) and single-strand deoxyribonucleic acid (ssDNA), chemically modified to protect their molecular integrity in physiological environment. Generally, aptamers fold into a specific tertiary structure and bind to targets such as proteins, metals, and molecules. The structural folding of oligonucleotides provides high affinity and excellent specificity toward targets, thus making them analogues of antibodies. Aptamers are generated by an in vitro molecular evolution method known as Systematic Evolution of Ligands by EXponential enrichment (SELEX) (72). The power of SELEX comes from the extremely large libraries of randomized nucleic acid sequences that can be efficiently screened for molecules with functional properties defined by the selection conditions. Such screening can be conducted against a variety of target molecules or elements, such as small compounds, proteins, nanoparticles, or live cells. Hence, aptamers can be used as reagents for affinity purification as well as detection (biosensors) and therapeutics to replace antibodies.

Stability of aptamers

Oligonucleotides are intrinsically unstable due to nucleolytic degradation by intracellular and extracellular nucleases. Such degradation is evaded by chemical modifications while the plasticity of the aptamer remains unaffected. Several options of modification are available and used to stabilise the aptamer. In addition to stability, it also turned out that finding high affinity aptamers against “difficult” human proteins were easier to find after modification (74).

Synthetic immune reagents - Nucleotide with modification options

Figure 7. Nucleotide with the options for chemical modification to stabilise the oligonucleotide aptamer. (Figure 2 from ref 73)

Aptamers for detection

The company SomaLogic developed a proteomic technology for biomarker discovery that is capable of simultaneously measuring more than a thousand proteins from small volumes of biological samples such as plasma, tissues, or cells. The technology is based on oligonucleotides with chemical modifications that greatly increase the physicochemical diversity of nucleic acid-based ligands. Such modifications introduce functional groups that are absent in natural nucleic acids but are often found in protein–protein, small molecule–protein, and antibody–antigen interactions. This new class of reagents are called SOMAmers (Slow Off-rate Modified Aptamers). The use of these modifications expands the range of possible targets for SELEX, results in improved binding properties, and facilitates selection of SOMAmers with slow dissociation rates (74). These slow off-rates in combination with the ability to hybridize to complementary sequences on a nucleic acid array have led to the development of the so-called SOMAscan.

Synthetic immune reagents - Schematic representation and sequence

Figure 8. (a) Schematic representation of an interaction between a SOMAmer (green) and its protein target (red). Modified residues at the interface are shown in purple. For the assay, the SOMAmer contains a biotin (B), a photocleavable linker (L), and a fluorophore (F) at the 5’end.

(b) Schematic sequence of assay steps leading to quantitative readout of target proteins (clockwise from top left): SOMAmer–protein binding, capture onto streptavidin-coated beads (“catch-1”), biotin-labeling of proteins, photocleavage followed by kinetic challenge, second biotin capture (“catch-2”), release from complexes into solution at high pH, and hybridization to complementary aptamer microarray. SOMAmers are detected by fluorescent tags when the array is scanned. (Figure 20.2 from ref 74)

This multiplex system benefits from high specificity due to a stringent wash (kinetic challenge, fig 8) with a polyanionic competitor that selectively disrupts non-cognate complexes. Because dissociation rates of cognate SOMAmer–protein interactions are much slower than those of nonspecific interactions. The quantity of each analyte is measured by the intensity of fluorescence on the microarray. Despite its complexity, the assay has a large overall dynamic range of 7 logs (from 100 fM to 1mM), low limits of detection (0.3 pM median), and excellent reproducibility (5% median intra-run and inter-run coefficient of variance).

This SOMAmer platform has been used successfully for biomarker discovery in a large set of diseases, and a detailed review was published recently (75). 

Aptamers for therapeutics

Aptamers could be used as carriers of therapy for accurate delivery of therapeutic drugs, such as chemotherapy drugs, small interfering RNAs, microRNAs, antisense oligos, or toxins, to the target cells but not to normal cells (76). These aptamer-drug conjugates (ApDCs) have gradually become effective drug delivery systems and attracted widespread attention (77). The simplest form of aptamer drug conjugation is non-covalent. Earlier aptamer-chemotherapy drug conjugates were constructed by non-covalent conjugation. Based on the various chemical properties of aptamers, many studies have proposed different aptamer-drug conjugation methods, such as covalent crosslinking, biocompatible spacers, cleavable linkers, and hydrophobic interaction. Examples are summarised in a new review (78).

Aptamer-mediated nucleic acid delivery for therapeutics is currently intensely tried and developed. Examples of siRNA delivery with reported success are: multivalent aptamer-siRNA conjugates (79), aptamer-based liposomes (80), bivalent aptamer-siRNA chimera (81) and multifunctional origami-based nanocarrier for codelivery of drugs and anti-sense oligonucleotides (128, 82).

Bispecific Aptamers have been developed, inspired by bispecific antibody therapies. Boltz et al. developed a stable nucleotide-based bispecific aptamer that mediates the lysis of c-Met-positive tumours, thus opening up new avenues for tumour therapy (83). In particular applications involving Natural Killer Cells have been developed (78).

Aptamers can be developed into cell growth factor mimics (84, 85) or extracellular matrix mimics (86, 87, 88), which can provide a favourable growth environment for cells, or simulate the related mechanisms in vivo, thus contributing to cell signal transduction and growth, as well as tissue repair.

The specificity and affinity of aptamers make them fit as mediators in cell recruitment for tissue repair or tumour targeting. Most repair studies focus on bone and cartilage tissue repair, in which the aptamers specific for Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs) are loaded onto the scaffold in bone therapy, and BM-MSCs are recruited to achieve regeneration therapy (89).  However, BM-MSCs are also key contributors to the tumour microenvironment (TME), and participate in tumour progression and promoting tumour metastasis by establishing a favourable TME (90). Hence, recruitment of BM-MSCs are prevented as an aptamer-mediated therapy through intervention of the platelet-derived growth factor receptor β (PDGFRβ). Camorani et al. used anti-PDGFRβ aptamer (Gint4.T) as a novel therapeutic tool to inhibit the homing of BM-MSCs to the triple-negative breast cancer (TNBC) microenvironment and TNBC-stromal interactions, ultimately interfering with BM-MSC-dependent pro-invasive activity (91).

To date, eleven aptamers have been clinically translated and are in various stages of clinical studies. The therapeutic scope of these aptamers includes eye disease, coagulation, oncology, and inflammation (reviewed in 78).

Selected recombinant Antibody suppliers

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GeneTex’s rAb protocol employs a multi-parameter FACS-based approach to isolate antigen-specific IgG+ memory B cells from an immunized animal. Once cloned, the supply of a given rAb is inexhaustible with exceptional reproducibility. supply of a given rAb is inexhaustible with exceptional reproducibility.
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Acknowledgments

This article was published first by Pivotal Scientific, The Life Sciences Consultancy. See https://pivotalscientific.com/scientific-library/synthetic-immune-reagents-as-alternatives-to-antibodies-raised-in-animals/