V5-tag and Nano-Trap
The V5-tag is a popular epitope tag derived from the P and V proteins of the Simian Virus 5 (SV5) that is used to tag proteins in yeast, bacteria, insect, and mammalian cells, allowing the detection and purification of the tagged protein. The V5-tag can be fused to the C-terminus or the N-terminus of the protein or be fused internally. Its amino acid sequence has a length of 14, GKPIPNPLLGLDST, with a molecular weight of 1.42 kDa.
Nano-Trap is ChromoTek's product line for the immunoprecipitation of tagged proteins. Their Nano-Trap line includes GFP-Trap, Myc-Trap, Spot-Trap, others, and since very recently, also V5-Trap. Nano-Trap differs from conventional affinity resin in the type of antibodies used: Nano-Trap antibodies are Nanobodies, very small antibodies derived from the heavy chain antibodies of camelids.
Molecular structure of V5-tag
Immunoprecipitation (IP) of V5-tagged proteins
While anti-V5-tag antibodies for conventional IP are available from multiple suppliers, ChromoTek is the first to offer anti-V5 Nanobodies bound to agarose beads for immunoprecipitation. V5-Trap is available as agarose or magnetic agarose.
Why should you use the V5-Trap?
Purity
V5-Trap contains Nanobodies instead of conventional antibodies, meaning you do not have to worry about heavy and light chains contaminating your eluted protein.
Flexibility
V5-Trap works for both C-terminus and N-terminus tagged proteins, giving you flexibility.
Low background
V5-Trap shows a very low protein background for cell lysates.
Efficiency
V5-Trap allows for a very efficient elution. Perform a gentle elution with V5-peptide, or use harsher methods with acidic conditions, both are possible with V5-Trap.
Compatibility
V5-Trap is compatible with downstream applications including mass spectrometric (MS) analysis, ELISA, and enzymatic assays.
High quality
Rigorously validated and characterized.