CRISPR Seminar Berne
Alt-R CRISPR-Cas9 - An optimized genome editing solution - Unleashing the Potential of Genetic Engineering
CRISPR technology has been a major technological breakthrough, making genome editing easier, faster, more efficient, and more robust than previous methods.
In this seminar you will learn:
- Guidelines when designing a CRISPR experiment.
- Advantages and disadvantages of Cas9 vs Cas12 for gene editing, and their applications.
- gRNA design and formats selection recommendation
- How to optimize cell-editing with high on-target and reduced off-target activity using Alt-R HiFi CRISPR-Cas9 nuclease.
- Considerations to improve HDR (Homology Directed Repair) efficiency for knock-in experiment.
- How chemical modifications for dsDNA donor templates improve the HDR efficiency and reduce blunt insertion events.
- How and where to introduce silent mutations to improve HDR rates
- How Alt-R HDR Enhancer V2 further improves HDR rate.
Speaker
Abdelilah Mekhloufi, Field Application Scientist, Integrated DNA Technologies
Host
Dr. Fitsumbirhan Mehari, Technical Sales Representative, LubioScience GmbH
When: May 10, 2023, 11.00 CEST
11:00 – 11:45 Seminar
11:45 – 12:30 Free lunch and discussion
Where: Berne (free)
Inselspital Bern, Kinderklinik A Nr. 181, Eingang 31 oder 31 B, Kursraum 1
Freiburgstrasse 15, 3010 Berne
Following the seminar, free lunch and drinks will be provided. We therefore kindly ask you to register.
The number of participants is limited. Hurry now to secure your place!