Webinar: CRISPR GUARD
Precise genome editing using CRISPR-Cas9 is a promising avenue for genetic disease research. However, there are a number of off-target editing issues to take into consideration.
Online
In this webinar, Dr. Matthew Coelho discusses findings from his recent publication in Nature Communications, "CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs."
You will learn:
- The utility of CRISPR Guide RNA Assisted Reduction of Damage (GUARD) as a method for protecting off-targets sites by co-delivery of short guide RNAs directed against off-target loci by competition with the on-target guide RNA.
- Project design rules and the versatility of this method for in vitro studies and high-throughput screening.
Speaker: Dr. Matthew Coelho, Postdoctoral Fellow, Wellcome Sanger Institute
Dr. Matthew Coelho is a postdoctoral researcher in the Garnett laboratory focusing on functional genomics strategies to understand molecular pathways involved in sensitivity to cancer immunotherapies. Dr. Coelho has gained prior expertise in cancer immunotherapy in the laboratory of Julian Downward at the Francis Crick Institute, as well as CRISPR and base editing technologies at AstraZeneca.
When: Thursday 19. June 2022 at 18:00 CET
Where: Online (free)