Webinar: From Chromatin Shearing to Leukaemia Discoveries
Join us while we share our newest insights and discoveries.
Online
Polycomb group proteins are epigenetic modifiers that are frequently deregulated in cancer and are known to regulate hematopoietic stem cell self-renewal and cell lineage specification. We previously reported that the non-canonical PRC1.1 Polycomb complex is essential for human leukemic stem cell maintenance (Van den Boom et al. Cell Reports, 2016; Maat et al. iScience, 2021). Paradoxically, loss-of-function mutations in the PRC1.1 subunits BCOR and BCORL1 can also drive AML development. To mimic the effects of BCOR/PRC1.1 loss-of-function, we generated mini auxin-inducible degron (mAID) knockin models for BCOR and the PRC1.1 chromatin-targeting subunit KDM2B in K562 leukemic cells. Using these models we have studied the immediate effects of BCOR and KDM2B loss on PRC1.1 complex integrity, investigated effects on the epigenetic landscape at PRC1.1 target genes, and evaluated transcriptional changes at these genes in a time-resolved manner.
Speakers:
Anne-Sophie Berthomieu: ChIP: the Gold-Standard for DNA/Protein Binding Profiling
Patricia Montilla: Introducing PIXUL sonication in your ChIP workflow
Vincent van den Booom: PRC1.1 loss-of-function transcriptional dynamics are wired in the epigenetic ground state of target genes
When: March 27, 2024, 16.00 CET
Where: Online (free)
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