Webinar: How to optimize non-viral CRISPR HDR for high-efficiency large knock-in in primary T cells and iPSCs

Achieving large knock-ins, such as chimeric antigen receptor (CAR) insertions in primary T lymphocytes, remains a key challenge in genome editing. In this webinar, we will present our findings from extensive bench research focused on optimizing CRISPR homology-directed repair (HDR) techniques in CAR knock-ins in primary T cells. We will cover crucial factors including double strand break repair mechanisms, CRISPR reagent selection, and optimized cell handling workflows. Additionally, we will highlight the use of Aldevron Nanoplasmids™ as highly efficient HDR donor templates, successfully demonstrated in both primary T cells and induced pluripotent stem cells (iPSCs). The session will conclude with a workflow summary and a Q&A session.

After this webinar, participants will be able to:

1. Understand principles of CRISPR: Gain a foundational understanding of CRISPR by comparing non-homologous end joining (NHEJ) and homology-directed repair (HDR).
2. Optimize primary T cell workflows: Learn best practices for activating and culturing primary T cells to enhance CRISPR editing efficiency.
3. Apply Nanoplasmids in HDR: Explore the benefits and optimization strategies for using Aldevron Nanoplasmids™ as donor templates for large insertions in CRISPR-mediated HDR.
4. Leverage HDR Enhancers: Learn how HDR enhancing reagents can boost editing success rates in challenging applications.

Speakers:

• Bernice Thommandru, MS: Research Scientist, Molecular Genetics Research Group, IDT
• Tyler Kozisek, PhD: Senior Scientist, Aldevron