AssayQuant delivers robust, easy-to-use, and high-value assays backed by innovative and proven technologies to enable your research efforts. With over 250 peer-reviewed publications and a dozen patents between them, they have unmatched expertise that's passed along to every customer.
AssayQuant's novel PhosphoSens® technology forms the basis for its extensive portfolio of more than 400 unique enzyme and inhibitor assays. The assays are available in two formats: The continuous assays are based on a straightforward measurement of fluorescence intensity while the red shift assays utilize time-resolved fluorescence. Both formats enable real-time monitoring of kinase activity, providing valuable insights for drug discovery and the development of new therapies.
Portfolio
- PhosphoSens-Red Kinase Assays
- PhosphoSens-Kinetic Kinase Assays
- PhophoSens-Kinetic Phosphatase Assays
Exclusive Phosphosens® Technology
AssayQuant’s patented PhosphoSens® Technology forms the basis of its extensive portfolio of more than 400 unique enzyme and inhibitor assays. Each assay features novel sensor-peptide substrates available in the form of a kit or bulk sensor-peptide and as a family of related standalone reagents, each component is tested carefully to ensure that it is optimized for use in the applicable assay. Bulk reagents are available at discounted pricing, for high throughput or larger volume applications.
Mode of Action
By harnessing chelation-enhanced fluorescence (ChEF) with improved sulfonamido-oxine (Sox) chromophore technology for peptide or protein substrates, we have created a simple yet powerful method to measure the activity of protein kinases or phosphatases using a direct and homogeneous format that provides more information with less effort and accommodates any ATP concentration or sample type.
The continuous (fluorescence intensity) format is ideal for elucidating drug mechanism of action, potency and profiling, while the red-shift (time resolved fluorescence) format is ideal for HTS and SAR. One can switch between formats with the same sensor, to provide seamless coverage across the entire discovery and drug development process.
- PhosphoSens: For purified enzymes. Kinetic or endpoint. Excitation Max 360 nm & Emission Max 485 nm, using fluorescence intensity.
- PhosphoSens-Lysate: Works the same as PhosphoSens but is designed for crude cell or tissue lysates.
- PhosphoSens-Red: For purified enzymes. Excitation Max 360 nm & Emission Max 620 nm, using Europium (Eu3+), time-resolved fluorescence (TRF) to eliminate interference due to compound autofluorescence.
The Advantages of PhosphoSens® Technology
- Reliable and Accurate - High accuracy & precision (Z’ > 0.7) and excellent sensor lot-to-lot consistency.
- Continuous - Captures the entire reaction in real-time enabling determination of kinetic parameters and detailed characterization of inhibition.
- Direct - Detects the actual event of phosphorylation and dephosphorylation rather than a proxy such as a binding event, ATP depletion, or ADP.
- Catalytic - PhosphoSens sensor peptides are optimized to bind to the substrate binding site enabling measurement of the true and complete catalytic process.
- Small, Minimally Hydrophobic - The Sox fluorophore is a fraction of the size of other fluorophores (comparable to the size of tryptophan) and is minimally hydrophobic to minimize artifacts.
- Versatile - Compatible with ATP Km for affinity and selectivity independent of compound MOI and physiological ATP (1-2mM) for continuity with cell-based assays.
- Physiologically Relevant - PhosphoSens sensor peptides are derived from and optimized for interaction with their target kinase or phosphatase.
- Novel and Non-Distruptive - Novel in its delivery of rich information in every well while being non-disruptive to your workflow (a simple add-and-read format) and equipment (standard fluorescence microplate reader).
