Kactus Biosystems

Check out our line of mammalian cell-derived MHC-I complexes including biotinylated and tetramer complexes.

Kactus provides a series of MHC-polypeptide complex proteins with natural conformation and complete glycosylation modification, including monomers, tetramers, biotinylated tetramers, VLPs, etc., to meet the requirements of immunization, antibody screening, TCRT, blocking assay, SPR and other applications.

T-Lymphocyte Activation by MHC

T lymphocytes, key mediators of the adaptive immune response, are activated when their T-cell receptors (TCRs) interact with cognate antigenic peptides displayed by major histocompatibility complex molecules (MHC).

Mammalian Expression

E. coli expression and in vitro protein refolding is a common method to produce MHC-I complexes. However, using our SAMS™ technology platform, KACTUS succeeded in expressing biotinylated MHC-I complex in the mammalian cell line Expi293.

High affinity, low endotoxin

The resulting MHC-I complexes show high affinities with benchmark antibodies and have low endotoxin levels – ideal for endotoxin sensitive cell-based assays. KACTUS also provides MHC tetramer complexes which have a greater avidity to T cells and more stable binding.

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