Lucigen - a LGC Biosearch Technologies company
Lucigen, part of LGC Biosearch Technologies, offers biomedical research products and services to customers worldwide, all under an ISO 13485 compliant quality system. Core competencies include enzyme and competent cell manufacture, next gen sequencing services and library preparation kits, to molecular diagnostics, applied, government and academic customers.
Products from Lucigen
- Competent Cells
- Nucleic acid extraction and purification
- Enzymes
- Array-based analysis
- CRISPR gene editing
- Cloning kits and vectors
- In vitro transcription
- Next generation sequencing kits
- PCR and amplification
- Protein expression
- Transposon mutagenesis
Competent cells
Lucigen a LGC Biosearch Technologies offers the best performance, value, and convenience in competent cells for cloning and expression. Our chemically competent cells offer an unmatched value for routine cloning, and our electrocompetent cells provide the highest possible efficiency for difficult cloning and library construction.
Lucigen offers a wide range of competent cells for:
- General cloning and library construction
- Phage display library applications
- CRISPR, large or difficult cloning
- Protein expression
- Low endotoxin cells
- Custom competent cells
TG1 Electrocompetent Cells
Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.
Endura Competent Cells
Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
10G Chemically or Electrocompetent Cells
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Nucleic acid extraction and purification
LGC Biosearch Technologies is unique in the nucleic acid extraction and purification marketplace, in that we are both a service provider, and a developer of proprietary chemistries and instrumentation. We develop chemistries and instrumentation for customers to use in their own laboratories, and it is these same products which we use in each of our service laboratories. This allows Biosearch Technologies to be highly flexible, and offer individual solutions for each of our customers.
QuickExtract - DNA Extraction Solution*
For DNA screening and genotyping applications, PCR-grade genomic DNA is sufficient, and can be rapidly and efficiently extracted using QuickExtract DNA Extraction Solution. In 3-8 minutes, and using only a single-tube protocol, PCR-grade genomic DNA or RNA from almost any sample type can be extracted, without the use of centrifugation, spin columns or any toxic organic solvent.
MasterPure™ - Complete DNA and RNA Purification Kit*
Some applications require flexibility of isolating high-molecular weight genomic DNA, total cellular RNA or total nucleic acid from the same sample. The MasterPure Complete DNA and RNA Purification Kit allows for isolation of NGS-grade DNA, RNA or total nucleic acid in 30-60 minutes using a single kit, and is suitable for many different sample types.
(*Products formerly known from Epicentre - an Illumina company)
Enzymes
Ligases, DNA polymerases, and RNA polymerases molecular biology enzymes with distrinct functions and applications:
Ligases
- NxGen T4 DNA Ligase
- Ampligase Thermostable DNA Ligase
- T4 RNA Ligase 2, Deletion Mutant
- CircLigase II ssDNA Ligase
- CircLigase ssDNA Ligase
DNA polymerases & RNA polymerases
- Bst DNA Polymerase, Exonuclease Minus
- NxGen phi29 DNA Polymerase
- Exo-Minus Klenow DNA Polymerase (D355A, E357A)
- FailSafe Enzyme Mix
- EconoTaq DNA Polymerase
- EconoTaq PLUS 2X Master Mix
- MasterAmp Tth DNA Polymerase
- NxGen T7 RNA Polymerase
- Poly(A) Polymerase Tailing Kit
- T7 R&DNA Polymerase
Reference
Hearn, R.P. and Arblaster, K.E. (2010), DNA extraction techniques for use in education. Biochem. Mol. Biol. Educ., 38: 161-166. https://doi.org/10.1002/bmb.20351
